tailieunhanh - Báo cáo khoa học: Drosophila melanogaster lipins are tissue-regulated and developmentally regulated and present specific subcellular distributions

Lipins constitute a novel family of Mg 2+ -dependent phosphatidate phos-phatases that catalyze the dephosphorylation of phosphatidic acid to yield diacylglycerol, an important intermediate in lipid metabolism and cell signaling. Whereas a single lipin is detected in less complex organisms, in mammals there are distinct lipin isoforms and paralogs that are differen-tially expressed among tissues. | ỊFEBS Journal Drosophila melanogaster lipins are tissue-regulated and developmentally regulated and present specific subcellular distributions Valeria Valente1 2 Rafaela Martins Maia1 Murilo Carlos Bizam Vianna1 and Maria Luisa Paọó-Larson1 1 Department of Cellular and Molecular Biology Ribeirao Preto Schoolof Medicine University of Sao Paulo Brazil 2 Department of Surgery and Anatomy Ribeirao Preto Schoolof Medicine University of Sao Paulo Brazil Keywords Drosophila isoforms lipin Pah1p subcellular localization Correspondence M. L. Paco-Larson Av. Bandeirantes 3900 Ribeirao Preto Sao Paulo Brazil Fax 55 16 36331786 Tel 55 16 36023347 E-mail mlplarso@ These authors contributed equally to this work Received 12 February 2010 revised 6 August 2010 accepted 9 September 2010 doi Lipins constitute a novel family of Mg2 -dependent phosphatidate phosphatases that catalyze the dephosphorylation of phosphatidic acid to yield diacylglycerol an important intermediate in lipid metabolism and cell signaling. Whereas a single lipin is detected in less complex organisms in mammals there are distinct lipin isoforms and paralogs that are differentially expressed among tissues. Compatible with organism tissue complexity we show that the single Drosophila Lpin1 ortholog CG8709 here named DmLpin expresses at least three isoforms DmLpinA DmLpinK and DmLpinJ in a temporal and spatially regulated manner. The highest levels of lipin in the fat body where DmLpinA and DmLpinK are expressed correlate with the highest levels of triacylglycerol TAG measured in this tissue. DmLpinK is the most abundant isoform in the central nervous system where TAG levels are significantly lower than in the fat body. In the testis where TAG levels are even lower DmLpinJ is the predominant isoform. Together these data suggest that DmLpinA might be the isoform that is mainly involved in TAG production and that DmLpinK and DmLpinJ could perform other cellular functions.