tailieunhanh - Báo cáo khoa học: Identification of carbonic anhydrase 9 as a contributor to pingyangmycin-induced drug resistance in human tongue cancer cells

Drug resistance is the major obstacle to successful cancer treatment. To understand the mechanisms responsible for drug resistance in tongue can-cer, Tca8113 cells derived from moderately differentiated human tongue squamous cell carcinoma were exposed to stepwise escalated concentrations of pingyangmycin (PYM) to develop the resistant cell line called Tca8113⁄ PYM | Identification of carbonic anhydrase 9 as a contributor to pingyangmycin-induced drug resistance in human tongue cancer cells Guopei Zheng1 z Min Zhou1 z Xinrong Ou2 Bo Peng1 Yanhui Yu1 Fangren Kong1 Yongmei Ouyang1 and Zhimin He1 1 Cancer Research Institute Xiangya Schoolof Medicine CentralSouth University Changsha Hunan China 2 Department of Stomatology Xiangya Hospital CentralSouth University Changsha Hunan China Keywords CA9 cDNA microarray drug resistance pingyangmycin tongue cancer Correspondence Zhimin He Cancer Research Institute Xiangya Schoolof Medicine CentralSouth University Xiangya Road 110 410078 Changsha Hunan China Fax 86 0731 82355043 Tel 86 0731 82355041 E-mail hezhimin2005@ These authors contributed equally to this work Received 20 June 2010 revised 6 August 2010 accepted 31 August 2010 doi Drug resistance is the major obstacle to successful cancer treatment. To understand the mechanisms responsible for drug resistance in tongue cancer Tca8113 cells derived from moderately differentiated human tongue squamous cell carcinoma were exposed to stepwise escalated concentrations of pingyangmycin PYM to develop the resistant cell line called Tca8113 PYM which showed over increased resistance to PYM as compared with Tca8113 cells and cross-resistance to cisplatin pirarubicin paclitaxel adriamycin and mitomycin. We found that the resistance was not associated with multidrug resistance transporter 1 p170 p-gp multidrug resistance-associated protein 1 and breast cancer resistance protein overexpression so we hypothesized that Tca8113 PYM cells must have some other resistance mechanism selected by PYM. To test this hypothesis the global gene expression profiles between Tca8113 and Tca8113 PYM cells were compared by cDNA microarray. Eighty-nine genes and thirteen expressed sequence tags with differential expression levels between the two cell lines were identified. Some differential expression levels were .