tailieunhanh - Báo cáo khoa học: Enzyme kinetics informatics: from instrument to browser

A limited number of publicly available resources provide access to enzyme kinetic parameters. These have been compiled through manual data mining of published papers, not from the original, raw experimental data from which the parameters were calculated. This is largely due to the lack of software or standards to support the capture, analysis, storage and dissemi-nation of such experimental data. | ỊFEBS Journal Enzyme kinetics informatics from instrument to browser Neil Swainston1 Martin Golebiewski2 Hanan L. Messiha1 Naglis Malys1 Renate Kania2 2 2 2 2 1 Sylvestre Kengne Olga Krebs Saqib Mir Heidrun Sauer-Danzwith Kieran Smallbone Andreas Weidemann2 Ulrike Wittig2 Douglas B. Kell1 Pedro Mendes1 3 Wolfgang Muller2 Norman W. Paton1 and Isabel Rojas2 1 Manchester Centre for Integrative Systems Biology University of Manchester UK 2 Heidelberg Institute for TheoreticalStudies Germany 3 Virginia Bioinformatics Institute Virginia Tech Blacksburg VA USA Keywords data analysis database enzyme kinetics metadata Correspondence N. Swainston Manchester Centre for Integrative Systems Biology University of Manchester Manchester M1 7DN UK Fax 44 161 306 8918 Tel 44 161 306 5146 E-mail Website http These authors contributed equally to this work Received 31 May 2010 revised 20 June 2010 accepted 13 July 2010 doi A limited number of publicly available resources provide access to enzyme kinetic parameters. These have been compiled through manual data mining of published papers not from the original raw experimental data from which the parameters were calculated. This is largely due to the lack of software or standards to support the capture analysis storage and dissemination of such experimental data. Introduced here is an integrative system to manage experimental enzyme kinetics data from instrument to browser. The approach is based on two interrelated databases the existing SABIO-RK database containing kinetic data and corresponding metadata and the newly introduced experimental raw data repository MeMo-RK. Both systems are publicly available by web browser and web service interfaces and are configurable to ensure privacy of unpublished data. Users of this system are provided with the ability to view both kinetic parameters and the experimental raw data from which they are calculated providing .