tailieunhanh - Báo cáo khoa học: High diversity of polyketide synthase genes and the melanin biosynthesis gene cluster in Penicillium marneffei

Despite the unique phenotypic properties and clinical importance ofPeni-cillium marneffei, the polyketide synthase genes in its genome have never been characterized. Twenty-three putative polyketide synthase genes and two putative polyketide synthase nonribosomal peptide-synthase hybrid genes were identified in theP. | High diversity of polyketide synthase genes and the melanin biosynthesis gene cluster in Penicillium marneffei Patrick C. Y. Woo1 2 3 Emily W. T. Tam3 Ken T. K. Chong3 James J. Cai3 Edward T. K. Tung3 Antonio H. Y. Ngan3 Susanna K. P. Lau1 2 3 and Kwok-Yung Yuen1 2 3 1 State Key Laboratory of Emerging Infectious Diseases Hong Kong China 2 Research Centre of Infection and Immunology The University of Hong Kong China 3 Department of Microbiology The University of Hong Kong China Keywords genome melanin Penicillium marneffei phylogeny polyketide synthase Correspondence . Yuen State Key Laboratory of Emerging Infectious Diseases Department of Microbiology The University of Hong Kong University Pathology Building Queen Mary Hospital Hong Kong China Fax 852 28551241 Tel 852 22554892 E-mail hkumicro@ Database Nucleotide sequence data of the 23 putative PKS genes and two putative PKS-NRPS genes are available in the EMBL GeriBarik DDBJ databases under the accession numbers HM070045-HM070069 Received 14 April 2010 revised 1 June 2010 accepted 13 July 2010 doi Despite the unique phenotypic properties and clinical importance of Peni-cillium marneffei the polyketide synthase genes in its genome have never been characterized. Twenty-three putative polyketide synthase genes and two putative polyketide synthase nonribosomal peptide-synthase hybrid genes were identified in the P. marneffei genome a diversity much higher than found in other pathogenic thermal dimorphic fungi such as Histoplas-ma capsulatum one polyketide synthase gene and Coccidioides immitis 10 polyketide synthase genes . These genes were evenly distributed on the phylogenetic tree with polyketide synthase genes of Aspergillus and other fungi indicating that the high diversity was not a result of lineage-specific gene expansion through recent gene duplication. The melanin-biosynthesis gene cluster had gene order and orientations identical to those in the Talaromy-ces