tailieunhanh - Báo cáo khoa học: Identification of a preferred substrate peptide for transglutaminase 3 and detection of in situ activity in skin and hair follicles

Transglutaminases (TGases) are a family of enzymes that catalyze cross-linking reactions between proteins. During epidermal differentiation, these enzymatic reactions are essential for formation of the cornified envelope, which consists of cross-linked structural proteins. Two main transglutamin-ases isoforms, epidermal-type (TGase 3) and keratinocyte-type (TGase 1), are cooperatively involved in this process of differentiating keratinocytes. | Identification of a preferred substrate peptide for transglutaminase 3 and detection of in situ activity in skin and hair follicles Asaka Yamane1 z Mina Fukui1 z Yoshiaki Sugimura1 Miho Itoh1 Mileidys Perez Alea2 Vincent Thomas2 Said El Alaoui2 Masashi Akiyama3 and Kiyotaka Hitomi1 1 Department of Applied Molecular Biosciences Graduate Schoolof BioagriculturalSciences Nagoya University Japan 2 CovalAb Villeurbanne France 3 Department of Dermatology Hokkaido University Graduate Schoolof Medicine Sapporo Japan Keywords epidermis hair follicle phage-display skin transglutaminase Correspondence K. Hitomi Department of Applied Molecular Biosciences Graduate School of BioagriculturalSciences Nagoya University Nagoya 464-8601 Japan Fax 81 52 789 5542 Tel 81 52 789 5541 E-mail hitomi@ These authors contributed equally to this work Received 13 May 2010 revised 4 July 2010 accepted 6 July 2010 doi Transglutaminases TGases are a family of enzymes that catalyze crosslinking reactions between proteins. During epidermal differentiation these enzymatic reactions are essential for formation of the cornified envelope which consists of cross-linked structural proteins. Two main transglutaminases isoforms epidermal-type TGase 3 and keratinocyte-type TGase 1 are cooperatively involved in this process of differentiating keratinocytes. Information regarding their substrate preference is of great importance to determine the functional role of these isozymes and clarify their possible co-operative action. Thus far we have identified highly reactive peptide sequences specifically recognized by TGases isozymes such as TGase 1 TGase 2 tissue-type isozyme and the blood coagulation isozyme Factor XIII. In this study several substrate peptide sequences for human TGase 3 were screened from a phage-displayed peptide library. The preferred substrate sequences for TGase 3 were selected and evaluated as fusion proteins with mutated glutathione .