tailieunhanh - báo cáo khoa học: " Novel exon combinations generated by alternative splicing of gene fragments mobilized by a CACTA transposon in Glycine max"

Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành y học dành cho các bạn tham khảo đề tài: Novel exon combinations generated by alternative splicing of gene fragments mobilized by a CACTA transposon in Glycine max | BMC Plant Biology BioMed Central Research article Open Access Novel exon combinations generated by alternative splicing of gene fragments mobilized by a CACTA transposon in Glycine max Gracia Zabala and Lila Vodkin Address Department of Crop Sciences University of Illinois Urbana Illinois 61801 USA Email Gracia Zabala - g-zabala@ LilaVodkin - l-vodkin@ Corresponding author Published 14 July 2007 Received 26 January 2007 BMC Plant Biology 2007 7 38 doi 1471-2229-7-38 Accepted I4 July 2007 This article is available from http 1471-2229 7 38 2007 Zabala and Vodkin licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License http licenses by which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Abstract Background The recent discoveries of transposable elements carrying host gene fragments such as the Pack-MULEs Mutator-like transposable elements of maize Zea mays rice Oryza sativa and Arabidopsis thaliana the Helitrons of maize and the Tgm-Express of soybeans revealed a widespread genetic mechanism with the potential to rearrange genomes and create novel chimeric genes affecting genomic and proteomic diversity. Not much is known with regard to the mechanisms of gene fragment capture by those transposon elements or the expression of the captured host gene fragments. There is some evidence that chimeric transcripts can be assembled and exist in EST collections. Results We report results obtained from analysis of RT-PCR derived cDNAs of the Glycine max mutant flower color gene wp that contains a transposon Tgm-Express1 in Intron 2 of the flavanone 3-hydroxylase gene F3H and is composed of five unrelated host gene fragments. The collection of cDNAs derived from the wp allele represents a multiplicity of processed RNAs varying in length and sequence that includes