tailieunhanh - báo cáo khoa học: " Frequency, type, and distribution of EST-SSRs from three genotypes of Lolium perenne, and their conservation across orthologous sequences of Festuca arundinacea, Brachypodium distachyon, and Oryza sativa"

Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành y học dành cho các bạn tham khảo đề tài: Frequency, type, and distribution of EST-SSRs from three genotypes of Lolium perenne, and their conservation across orthologous sequences of Festuca arundinacea, Brachypodium distachyon, and Oryza sativa | BMC Plant Biology BioMed Central Research article Open Access Frequency type and distribution of EST-SSRs from three genotypes of Lolium perenne and their conservation across orthologous sequences of Festuca arundinacea Brachypodium distachyon and Oryza sativa Torben Asp 1 Ursula K Frei1 Thomas Didion2 Klaus K Nielsen2 and Thomas Lubberstedt1 Address Department of Genetics and Biotechnology University of Arhus Research Centre Flakkebjerg Fors0gsvej 1 4200 Slagelse Denmark and 2DLF-Trifolium Ltd. Research Division 4660 Store Heddinge Denmark Email Torben Asp - Ursula K Frei - Thomas Didion - tdi@ Klaus K Nielsen - kkn@ Thomas Lubberstedt - Corresponding author Published 12 July 2007 BMC Plant Biology 2007 7 36 doi 1471-2229-7-36 Received 5 March 2007 Accepted 12 July 2007 This article is available from http 1471-2229 7 36 2007 Asp et al licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License http licenses by which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Abstract Background Simple sequence repeat SSR markers are highly informative and widely used for genetic and breeding studies in several plant species. They are used for cultivar identification variety protection as anchor markers in genetic mapping and in marker-assisted breeding. Currently a limited number of SSR markers are publicly available for perennial ryegrass Lolium perenne . We report on the exploitation of a comprehensive EST collection in L. perenne for SSR identification. The objectives of this study were 1 to analyse the frequency type and distribution of SSR motifs in ESTs derived from three genotypes of L. perenne 2 to perform a comparative analysis of SSR motif polymorphisms between allelic sequences 3 to conduct a