tailieunhanh - Calcium-sensing receptor mediates phenylalanine-induced cholecystokinin secretion in enteroendocrine STC-1 cellsTohru Hira1, Shingo Nakajima2, Yuzuru Eto3 and Hiroshi Hara11 Division of Applied Biosciences, Research Faculty of Agriculture, Hokkaido Univ

Intraluminall-phenylalanine (Phe) stimulates cholecystokinin (CCK) secre-tion in vivo andin vitro. However, the cellular mechanism by which CCK-producing enteroendocrine cells sense Phe is unknown. The calcium-sensing receptor (CaR) can sense amino acids, and is expressed in the gastrointesti-nal tract. | ễFEBS Journal Calcium-sensing receptor mediates phenylalanine-induced cholecystokinin secretion in enteroendocrine STC-1 cells Tohru Hira1 Shingo Nakajima2 Yuzuru Eto3 and Hiroshi Hara1 1 Division of Applied Biosciences Research Faculty of Agriculture Hokkaido University Sapporo Japan 2 Division of Bio-systems and Sustainability Graduate Schoolof Agriculture Hokkaido University Sapporo Japan 3 Institute of Life Sciences Ajinomoto Co. Inc. Kawasaki Japan Keywords calcium-sensing receptor cholecystokinin enteroendocrine cells phenylalanine STC-1 cells Correspondence T. Hira Division of Applied Biosciences Research Faculty of Agriculture Hokkaido University Kita-9 Nishi-9 Kita-ku Sapporo 060-8589 Japan Fax 81 11 706 2504 Tel 81 11 706 2811 E-mail hira@ Received 23 May 2008 revised 22 July 2008 accepted 22 July 2008 doi Intraluminal L-phenylalanine Phe stimulates cholecystokinin CCK secretion in vivo and in vitro. However the cellular mechanism by which CCK-producing enteroendocrine cells sense Phe is unknown. The calcium-sensing receptor CaR can sense amino acids and is expressed in the gastrointestinal tract. In the present study we examined whether CaR functions as a receptor for Phe in CCK-producing enteroendocrine cells. CCK secretion and intracellular Ca2 concentration in response to Phe were measured in the murine CCK-producing enteroendocrine cell line STC-1 at various extracellular Ca2 concentrations or after treatment with a CaR antagonist. At more than 20 mM Phe induced dose-dependent CCK secretion and intracellular Ca2 mobilization in STC-1 cells. In the presence of mM extracellular Ca2 10 and 20 mM Phe induced significantly higher CCK secretion than under normal conditions mM extracellular Ca2 . Intracellular Ca2 mobilization induced by 10 or 20 mM Phe was also enhanced by increasing extracellular Ca2 concentrations. In addition intracellular Ca2 mobilization induced by addition of .

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