tailieunhanh - Báo cáo khoa học: Hodgkin Reed–Sternberg cells express 15-lipoxygenase-1 and are putative producers of eoxins in vivo Novel insight into the inflammatory features of classical Hodgkin lymphoma

Classical Hodgkin lymphoma has unique clinical and pathological features and tumour tissue is characterized by a minority of malignant Hodgkin Reed–Sternberg cells surrounded by inflammatory cells. In the present study, we report that the Hodgkin lymphoma-derived cell line L1236 has high expression of 15-lipoxygenase-1 and that these cells readily convert arachidonic acid to eoxin C4, eoxin D4and eoxin E4. | ễFEBS Journal Hodgkin Reed-Sternberg cells express 15-lipoxygenase-1 and are putative producers of eoxins in vivo Novel insight into the inflammatory features of classical Hodgkin lymphoma Hans-Erik Claesson1 2 William J. Griffiths3 Asa Brunnstrom2 Frida Schain4 Erik Andersson2 4 Stina Feltenmark1 2 Helene A. Johnson1 Anna Porwit5 Jan Sjoberg4 and Magnus Bjorkholm4 1 Department of MedicalBiochemistry and Biophysics Karolinska Institutet Stockholm Sweden 2 Orexo AB Uppsala Sweden 3 Institute of Mass Spectrometry Schoolof Medicine Swansea University UK 4 Division of Hematology Department of Medicine Karolinska University Hospitaland Institutet Stockholm Sweden 5 Department of Pathology Karolinska University Hospitaland Institutet Stockholm Sweden Keywords arachidonic acid Hodgkin lymphoma inflammation L1236 lipoxygenase Correspondence . Claesson Department of Medical Biochemistry and Biophysics Division of PhysiologicalChemistry II Karolinska Institutet SE-171 77 Stockholm Sweden Fax 46 8 324 264 Tel 46 8 524 87627 E-mail Received 14 April2008 revised 18 June 2008 accepted 23 June 2008 doi Classical Hodgkin lymphoma has unique clinical and pathological features and tumour tissue is characterized by a minority of malignant Hodgkin Reed-Sternberg cells surrounded by inflammatory cells. In the present study we report that the Hodgkin lymphoma-derived cell line L1236 has high expression of 15-lipoxygenase-1 and that these cells readily convert arachidonic acid to eoxin C4 eoxin D4 and eoxin E4. These mediators were only recently discovered in human eosinophils and mast cells and found to be potent proinflammatory mediators. Western blot and immunocytochemistry analyses of L1236 cells demonstrated that 15-lipoxygenase-1 was present mainly in the cytosol and that the enzyme translocated to the membrane upon calcium challenge. By immunohistochemistry of Hodgkin lymphoma tumour tissue 15-lipoxygenase-1 was found .