tailieunhanh - Báo cáo khoa học: Truncated P-cadherin is produced in oral squamous cell carcinoma

Cadherins belong to a family of homophilic cell–cell adhesion proteins that are responsible for the establishment of a precise cell architecture and tissue integrity. Moreover, experimental data suggest that loss of intercellular adhesion is inversely correlated with cellular differentiation. Furthermore, dedifferentiation is closely linked to tumor progression. | ễFEBS Journal Truncated P-cadherin is produced in oral squamous cell carcinoma Richard Bauer1 Albert Dowejko1 Oliver Driemel1 . BoBerhoff2 and T. E. Reichert1 1 Department of Oraland MaxillofacialSurgery University of Regensburg Germany 2 Institute of Pathology University of Regensburg Germany Keywords cell adhesion keratinocytes migration oral squamous cell carcinoma truncated P-cadherin Correspondence R. Bauer Department of Oral and MaxillofacialSurgery University of Regensburg Franz-Josef-Strauss-Allee 11 93053 Regensburg Germany Fax 49 943 1631 Tel 49 941 943 1627 E-mail Received 21 April2008 revised 12 June 2008 accepted 23 June 2008 doi Cadherins belong to a family of homophilic cell-cell adhesion proteins that are responsible for the establishment of a precise cell architecture and tissue integrity. Moreover experimental data suggest that loss of intercellular adhesion is inversely correlated with cellular differentiation. Furthermore dedifferentiation is closely linked to tumor progression. Recently we have shown that a secreted 50 kDa N-terminal fragment of P-cadherin plays a role in the progression of malignant melanoma. In this study we have detected both the full-length and the truncated versions of P-cadherin in cell lysates of differentiated head and neck oral squamous cell carcinoma cell lines whereas in cell lysates of dedifferentiated cell lines we detected only the truncated 50 kDa version of P-cadherin. Treatment of the cell lines with a recombinantly expressed biotinylated soluble 50 kDa form of the N-terminal part of P-cadherin revealed a major effect on cell aggregation and migration of oral squamous cell carcinoma cells. However the 50 kDa N-terminal fragment of P-cadherin did not show any influence on cell proliferation in 2D and 3D cell culture. These results suggest that generation of truncated P-cadherin during the progression of oral squamous carcinoma attenuates