tailieunhanh - báo cáo khoa học: "Development of new genomic microsatellite markers from robusta coffee (Coffea canephora Pierre ex A. Froehner) showing broad cross-species transferability and utility in genetic studies"

Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành y học dành cho các bạn tham khảo đề tài: Development of new genomic microsatellite markers from robusta coffee (Coffea canephora Pierre ex A. Froehner) showing broad cross-species transferability and utility in genetic studies | BMC Plant Biology BioMed Central Open Access Research article Development of new genomic microsatellite markers from robusta coffee Coffea canephora Pierre ex A. Froehner showing broad cross-species transferability and utility in genetic studies Prasad Suresh Hendre Regur Phanindranath V Annapurna Albert Lalremruata and Ramesh K Aggarwal Address Centre for Cellular and Molecular Biology CCMB Uppal Road Tarnaka Hyderabad- 500 007 Andhra Pradesh India Email Prasad Suresh Hendre - prasadhendre@ Regur Phanindranath - phanindra@ V Annapurna - purnavneni@ Albert Lalremruata - Ramesh K Aggarwal - rameshka@ Corresponding author Published 30 April 2008 Received 27 September 2007 BMC Plant Biology 2008 8 51 doi 1471-2229-8-51 Accepted 30 April 2008 This article is available from http 1471-2229 8 51 2008 Hendre et al licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License http licenses by which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Abstract Background Species-specific microsatellite markers are desirable for genetic studies and to harness the potential of MAS-based breeding for genetic improvement. Limited availability of such markers for coffee one of the most important beverage tree crops warrants newer efforts to develop additional microsatellite markers that can be effectively deployed in genetic analysis and coffee improvement programs. The present study aimed to develop new coffee-specific SSR markers and validate their utility in analysis of genetic diversity individualization linkage mapping and transferability for use in other related taxa. Results A small-insert partial genomic library of Coffea canephora was probed for various SSR motifs following conventional approach of Southern hybridisation. .