tailieunhanh - báo cáo khoa học: " Transcriptional profiling of Medicago truncatula meristematic root cells"

Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành y học dành cho các bạn tham khảo đề tài: Transcriptional profiling of Medicago truncatula meristematic root cells | BMC Plant Biology BioMed Central Research article Open Access Transcriptional profiling of Medicago truncatula meristematic root cells Peta Holmes1 Nicolas Goffard1 2 Georg F Weiller1 Barry G Rolfe1 and Nijat Imin 1 Address 1ARC Centre of Excellence for Integrative Legume Research Genomic Interactions Group Research School of Biological Sciences Australian National University Canberra ACT 2601 Australia and 2Institut Louis Malardé GP Box 30 98713 Papeete Tahiti French Polynesia Email Peta Holmes - Nicolas Goffard - ngoffard@ Georg F Weiller - Barry G Rolfe - Nijat Imin - Corresponding author Published 27 February 2008 Received 15 June 2007 BMC Plant Biology 2008 8 21 doi l47l-2229-8-2l Accepted 27 February 2008 This article is available from http l47l-2229 8 2l 2008 Holmes et al licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License http licenses by which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Abstract Background The root apical meristem of crop and model legume Medicago truncatula is a significantly different stem cell system to that of the widely studied model plant species Arabidopsis thaliana. In this study we used the Affymetrix Medicago GeneChip to compare the transcriptomes of meristem and non-meristematic root to identify root meristem specific candidate genes. Results Using mRNA from root meristem and non-meristem we were able to identify 324 and 363 transcripts differentially expressed from the two regions. With bioinformatics tools developed to functionally annotate the Medicago genome array we could identify significant changes in metabolism signalling and the differentially expression of 55 transcription factors in meristematic and non-meristematic .

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