tailieunhanh - báo cáo khoa học: " A high-throughput screening system for barley/powdery mildew interactions based on automated analysis of light micrographs"

Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành y học dành cho các bạn tham khảo đề tài: A high-throughput screening system for barley/powdery mildew interactions based on automated analysis of light micrographs | BMC Plant Biology BioMed Central Research article Open Access A high-throughput screening system for barley powdery mildew interactions based on automated analysis of light micrographs Alexander Ihlow 1 2 Patrick Schweizer2 and Udo Seiffert1 Address 1Pattern Recognition Group IPK Gatersleben Corrensstr. 3 D-06466 Gatersleben Germany and 2Transcriptome Analysis Group IPK Gatersleben Corrensstr. 3 D-06466 Gatersleben Germany Email Alexander Ihlow - ihlow@ Patrick Schweizer - schweiz@ Udo Seifert - seiffert@ Corresponding author Published 23 January 2008 Received 13 August 2007 BMC Plant Biology 2008 8 6 doi 1471 -2229-8-6 Accepted 23 January 2008 This article is available from http 1471-2229 8 6 2008 Ihlow et al licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License http licenses by which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Abstract__ Background To find candidate genes that potentially influence the susceptibility or resistance of crop plants to powdery mildew fungi an assay system based on transient-induced gene silencing TIGS as well as transient over-expression in single epidermal cells of barley has been developed. However this system relies on quantitative microscopic analysis of the barley powdery mildew interaction and will only become a high-throughput tool of phenomics upon automation of the most time-consuming steps. Results We have developed a high-throughput screening system based on a motorized microscope which evaluates the specimens fully automatically. A large-scale double-blind verification of the system showed an excellent agreement of manual and automated analysis and proved the system to work dependably. .

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