tailieunhanh - báo cáo khoa học: " Quantitative 1H NMR metabolomics reveals extensive metabolic reprogramming of primary and secondary metabolism in elicitor-treated opium poppy cell cultures"

Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành y học dành cho các bạn tham khảo đề tài: Quantitative 1H NMR metabolomics reveals extensive metabolic reprogramming of primary and secondary metabolism in elicitor-treated opium poppy cell cultures | BMC Plant Biology BioMed Central Open Access Research article Quantitative H NMR metabolomics reveals extensive metabolic reprogramming of primary and secondary metabolism in elicitor-treated opium poppy cell cultures Katherine G Zulak Aalim M Weljie Hans J Vogel and Peter J Facchini Address Department of Biological Sciences University of Calgary Calgary Alberta T2N 1N4 Canada Email Katherine G Zulak - zulakk@ Aalim M Weljie - aweljie@ Hans J Vogel - vogel@ Peter J Facchini - pfacchin@ Corresponding author Published 22 January 2008 Received 19 September 2007 BMC Plant Biology 2008 8 5 doi 1471 -2229-8-5 Accepted 22 January 2008 This article is available from http l47l-2229 8 5 2008 Zulak et al licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License http licenses by which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Abstract Background Opium poppy Papaver somniferum produces a diverse array of bioactive benzylisoquinoline alkaloids and has emerged as a model system to study plant alkaloid metabolism. The plant is cultivated as the only commercial source of the narcotic analgesics morphine and codeine but also produces many other alkaloids including the antimicrobial agent sanguinarine. Modulations in plant secondary metabolism as a result of environmental perturbations are often associated with the altered regulation of other metabolic pathways. As a key component of our functional genomics platform for opium poppy we have used proton nuclear magnetic resonance H NMR metabolomics to investigate the interplay between primary and secondary metabolism in cultured opium poppy cells treated with a fungal elicitor. Results Metabolite fingerprinting and compound-specific profiling showed the extensive reprogramming of primary .