tailieunhanh - báo cáo khoa học: " Characterization of expressed sequence tags obtained by SSH during somatic embryogenesis in Cichorium intybus L"

Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành y học dành cho các bạn tham khảo đề tài: Characterization of expressed sequence tags obtained by SSH during somatic embryogenesis in Cichorium intybus L | BMC Plant Biology BioMed Central Research article Open Access Characterization of expressed sequence tags obtained by SSH during somatic embryogenesis in Cichorium intybus L Sylvain Legrand Theo Hendriks Jean-Louis Hilbert and Marie-Christine Quillet Address UMR USTL INRA 1281 Stress Abiotiques et Différenciation des Végétaux Cultivés Université de Sciences et Technologies de LILLE Bâtiment SN2 59655 Villeneuve d Ascq France Email Sylvain Theo Jean-Louis Hilbert - jean- Marie-Christine Quillet - Corresponding author Published 6 June 2007 Received 10 August 2006 BMC Plant Biology 2007 7 27 doi 1471-2229-7-27 Accepted 6 June 2007 This article is available from http 1471-2229 7 27 2007 Legrand et al licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License http licenses by which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Abstract Background Somatic embryogenesis SE is an asexual propagation pathway requiring a somatic-to-embryonic transition of differentiated somatic cells toward embryogenic cells capable of producing embryos in a process resembling zygotic embryogenesis. In chicory genetic variability with respect to the formation of somatic embryos was detected between plants from a population of Cichorium intybus L. landrace Koospol. Though all plants from this population were self incompatible we managed by repeated selfing to obtain a few seeds from one highly embryogenic E plant K59. Among the plants grown from these seeds one plant C15 was found to be non-embryogenic NE under our SE-inducing conditions. Being closely related we decided to exploit the difference in SE capacity between K59 and its descendant C15 to study .

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