tailieunhanh - báo cáo khoa học: " Impact of AtNHX1, a vacuolar Na+/H+ antiporter, upon gene expression during short- and long-term salt stress in Arabidopsis thaliana"

Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành y học dành cho các bạn tham khảo đề tài: Impact of AtNHX1, a vacuolar Na+/H+ antiporter, upon gene expression during short- and long-term salt stress in Arabidopsis thaliana | BMC Plant Biology BioMed Central Research article Impact of AtNHXl a vacuolar Na H antiporter upon gene expression during short- and long-term salt stress in Arabidopsis thaliana Jordan B Sottosanto Yehoshua Saranga and Eduardo Blumwald Open Access Address Department of Plant Sciences University of California One Shields Ave Davis CA 95616 USA Email Jordan B Sottosanto - jbso@ Yehoshua Saranga - saranga@ Eduardo Blumwald - eblumwald@ Corresponding author Published 5 April 2007 Received 12 August 2006 BMC Plant Biology 2007 7 18 doi 1471-2229-7-18 Accepted 5 April 2007 This article is available from http 1471-2229 7 18 2007 Sottosanto et al licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License http licenses by which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Abstract Background AtNHX1 the most abundant vacuolar Na H antiporter in Arabidopsis thaliana mediates the transport of Na and K into the vacuole influencing plant development and contributing to salt tolerance. In this report microarray expression profiles of wild type plants a T-DNA insertion knockout mutant of AtNHX nhx and a rescued line NHX1 nhx were exposed to both short 12 h and 48 h and long one and two weeks durations of a non-lethal salt stress to identify key gene transcripts associated with the salt response that are influenced by AtNHX . Results 147 transcripts showed both salt responsiveness and a significant influence of AtNHX1. Fifty-seven of these genes showed an influence of the antiporter across all salt treatments while the remaining genes were influenced as a result of a particular duration of salt stress. Most 69 of the genes were up-regulated in the absence of AtNHX1 with the exception of transcripts encoding proteins involved with metabolic and energy