tailieunhanh - Báo cáo khoa học: Role of different moieties from the lipooligosaccharide molecule in biological activities of the Moraxella catarrhalis outer membrane

Lipooligosaccharide (LOS), a major component of the outer membrane of Moraxella catarrhalis, consists of two major moieties: a lipid A and a core oligosaccharide (OS). The core OS can be dissected into a linker and three OS chains. | ễFEBS Journal Role of different moieties from the lipooligosaccharide molecule in biological activities of the Moraxella catarrhalis outer membrane Daxin Peng1 z Wei-Gang Hu1 t Biswa P. Choudhury2 Artur Muszynski2 Russell W. Carlson2 and Xin-Xing Gu1 1 Vaccine Research Section National institute on Deafness and Other Communication Disorders National institutes of Health Rockville MD USA 2 Complex Carbohydrate Research Center University of Georgia Athens GA USA Keywords lipooligosaccharide moiety Moraxella catarrhalis outer membrane role Correspondence Xin-Xing Gu 5 Research Court Room 2A31 Rockville MD 20850 USA Fax 1 301 435 4040 Tel 1 301 402 2456 E-mail guxx@ Present addresses Schoolof Veterinary Medicine Yangzhou University Yangzhou Jiangsu China TDefence Research and Development Canada-Suffield Medicine Hat Alberta Canada Database The nucleotide sequence of lgt3 gene in Moraxella catarrhalis strain O35E has been submitted to the GenBank database under the accession number DQ208195 Lipooligosaccharide LOS a major component of the outer membrane of Moraxella catarrhalis consists of two major moieties a lipid A and a core oligosaccharide OS . The core OS can be dissected into a linker and three OS chains. To gain an insight into the biological activities of the LOS molecules of M. catarrhalis we used a random transposon mutagenesis approach with an LOS specific monoclonal antibody to construct a serotype A O35Elgt3 LOS mutant. MALDI-TOF-MS of de-O-acylated LOS from the mutant and glycosyl composition linkage and NMR analysis of its OS indicated that the LOS contained a truncated core OS and consisted of a Glc-Kdo2 linker -lipid A structure. Phenotypic analysis revealed that the mutant was similar to the wild-type strain in its growth rate toxicity and susceptibility to hydrophobic reagents. However the mutant was sensitive to bactericidal activity of normal human serum and had a reduced adherence to human epithelial cells. These data combined with .

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