tailieunhanh - báo cáo khoa học: " Rapid and accurate pyrosequencing of angiosperm plastid genomes"

Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành y học dành cho các bạn tham khảo đề tài: Rapid and accurate pyrosequencing of angiosperm plastid genomes | BMC Plant Biology BioMed Central Research article Open Access Rapid and accurate pyrosequencing of angiosperm plastid genomes Michael J Moore 1 2 Amit Dhingra3 Pamela S Soltis2 Regina Shaw4 William G Farmerie4 Kevin M Folta3 and Douglas E Soltis1 Address Department of Botany University of Florida . Box 118526 Gainesville FL 32611 USA 2Florida Museum of Natural History University of Florida . Box 117800 Gainesville FL 32611 USA 3Horticultural Sciences Department University of Florida . Box 110690 Gainesville FL 32611 USA and 4ICBR Genome Sequencing Service Laboratory University of Florida . Box 100156 Gainesville FL 32610 USA Email Michael J Moore - mjmoore1@ Amit Dhingra - adhingra@ Pamela S Soltis - psoltis@ Regina Shaw - regina@ William G Farmerie - wgf@ Kevin M Folta - kfolta@ Douglas E Soltis - dsoltis@ Corresponding author Published 25 August 2006 Received 06 April 2006 BMC Plant Biology 2006 6 17 doi 1471-2229-6-17 Accepted 25 August 2006 This article is available from http 1471-2229 6 17 2006 Moore et al licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License http licenses by which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Abstract Background Plastid genome sequence information is vital to several disciplines in plant biology including phylogenetics and molecular biology. The past five years have witnessed a dramatic increase in the number of completely sequenced plastid genomes fuelled largely by advances in conventional Sanger sequencing technology. Here we report a further significant reduction in time and cost for plastid genome sequencing through the successful use of a newly available pyrosequencing platform the Genome Sequencer 20 GS 20 System 454 Life .