tailieunhanh - Báo cáo y học: " Picoeukaryotic sequences in the Sargasso Sea metagenome"

Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học Critical Care giúp cho các bạn có thêm kiến thức về ngành y học đề tài: Picoeukaryotic sequences in the Sargasso Sea metagenome. | Research Open Access Picoeukaryotic sequences in the Sargasso Sea metagenome Gwenael Piganeau Yves Desdevises Evelyne Derelle and Herve Moreau Addresses UPMC Univ Paris 06 UMR 7628 MBCE Observatoire Océanologique F-66651 Banyuls mer France. ÝCNRS UMR 7628 MBCE Observatoire Océanologique F-66651 Banyuls mer France. Correspondence Gwenael Piganeau. Email Published 7 January 2008 Genome Biology 2008 9 R5 doi gb-2008-9-l-r5 The electronic version of this article is the complete one and can be found online at http 2008 9 1 R5 Received 16 October 2007 Revised 6 December 2007 Accepted 7 January 2008 2008 Piganeau et al. licensee BioMed Central Ltd. This is an open access article distributed under the terms of the Creative Commons Attribution License http licenses by which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Abstract Background With genome sequencing becoming more and more affordable environmental shotgun sequencing of the microorganisms present in an environment generates a challenging amount of sequence data for the scientific community. These sequence data enable the diversity of the microbial world and the metabolic pathways within an environment to be investigated a previously unthinkable achievement when using traditional approaches. DNA sequence data assembled from extracts of pm filtered Sargasso seawater unveiled an unprecedented glimpse of marine prokaryotic diversity and gene content. Serendipitously many sequences representing picoeukaryotes cell size 2 pm were also present within this dataset. We investigated the picoeukaryotic diversity of this database by searching sequences containing homologs of eight nuclear anchor genes that are well conserved throughout the eukaryotic lineage as well as one chloroplastic and one mitochondrial gene. Results We found up to 41 distinct eukaryotic scaffolds with