tailieunhanh - Atomic Force Microscopy Episode 2 Part 7

Tham khảo tài liệu 'atomic force microscopy episode 2 part 7', kỹ thuật - công nghệ, cơ khí - chế tạo máy phục vụ nhu cầu học tập, nghiên cứu và làm việc hiệu quả | 328 Grimaldi et al. Fig. 1. Constant force AFM images of untreated A 10 X 10 pm and respectively 9 h B 13 X 13 pm 24 h C 14 X 14 pm and 64 h D 13 X 13 pm -exposed Raji cells. The gray scale is defined so that lighter colors correspond to higher corrugations. It is worth noting that the top height reported for the cells progressively decreases at increasing exposure. In A microvilli are visible as a lighter spot on the cell membrane that are no more recognizable after 9-15 h B . At very long exposure the cell surface become characterized by several furrows and infolding D . A cross-section of these cells taken along the white line A-A is shown in Fig. 4. border of the cells anchoring structures named pseudopodia that are similarly to microvilli involved in cell migration. Cells exposed for 9 h Fig. 1B and 2B to MF show clear differences compared to untreated cells in this case the microvilli have almost completely disappeared from the surface of the cells and also pseudopodia are hardly detectable. Lymphoblastoid Cells and Low-Frequency Magnetic Fields 329 A B c D Fig. 2. Lateral friction AFM images of the same cells shown in Figs. 1A-D respectively. Lateral friction images collected simultaneously to topography are very sensitive to small structures protruding from a large and corrugated surface so that they are very suitable for describing microvilli A as well as the fine surface modifications induced by MF exposure for instance the pits structure in D . . Cell Membrane Features The cell membrane which because of the microvilli could not be directly observed in untreated samples appears quite smooth with no structures or protrusions on the surface. In cells exposed for 15 h to MF not shown both microvilli and pseudopodia can no longer be recognized whereas as in samples 330 Grimaldi et al. exposed for a shorter time the dome shape is essentially unchanged. After 24 h of exposure to MF Figs. 1C and 2C a slow membrane change is still going on as revealed by the .

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