tailieunhanh - Báo cáo y học: "Widespread duplications in the genomes of laboratory stocks of Dictyostelium discoideu"

Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học Critical Care giúp cho các bạn có thêm kiến thức về ngành y học đề tài: Widespread duplications in the genomes of laboratory stocks of Dictyostelium discoideum. | Open Access Widespread duplications in the genomes of laboratory stocks of Dictyostelium discoideum Gareth Bloomfield Yoshimasa Tanaka Jason Skelton Alasdair Ivens and Robert R Kay Addresses MRC Laboratory of Molecular Biology Hills Road Cambridge CB2 0QH UK. The Wellcome Trust Sanger Institute Wellcome Trust Genome Campus Hinxton Cambridge CB10 1SA UK. Correspondence Gareth Bloomfield. Email garethb@ Published 22 April 2008 Genome Biology 2008 9 R75 doi 186 gb-2008-9-4-r75 The electronic version of this article is the complete one and can be found online at http 2008 9 4 R75 Received 19 December 2007 Revised 19 March 2008 Accepted 22 April 2008 2008 Bloomfield et al. licensee BioMed Central Ltd. This is an open access article distributed under the terms of the Creative Commons Attribution License http licenses by which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Abstract Background Duplications of stretches of the genome are an important source of individual genetic variation but their unrecognized presence in laboratory organisms would be a confounding variable for genetic analysis. Results We report here that duplications of 15 kb or more are common in the genome of the social amoeba Dictyostelium discoideum. Most stocks of the axenic workhorse strains Ax2 and Ax3 4 obtained from different laboratories can be expected to carry different duplications. The auxotrophic strains DH1 and JH10 also bear previously unreported duplications. Strain Ax3 4 is known to carry a large duplication on chromosome 2 and this structure shows evidence of continuing instability we find a further variable duplication on chromosome 5. These duplications are lacking in Ax2 which has instead a small duplication on chromosome 1. Stocks of the type isolate NC4 are similarly variable though we have identified some approximating the assumed ancestral .