tailieunhanh - Báo cáo y học: "Erect wing regulates synaptic growth in Drosophila by integration of multiple signaling pathway"

Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học Critical Care giúp cho các bạn có thêm kiến thức về ngành y học đề tài: Erect wing regulates synaptic growth in Drosophila by integration of multiple signaling pathways. | Research Open Access Erect wing regulates synaptic growth in Drosophila by integration of multiple signaling pathways Irmgard U Haussmann Kalpana White and Matthias Soller Addresses School of Biosciences University of Birmingham Edgbaston Birmingham B15 2TT UK. Department of Biology and Volen Center for Complex Systems Brandeis University Waltham MA 02454 USA. Correspondence Matthias Soller. Email Published 17 April 2008 Genome Biology 2008 9 R73 doi 186 gb-2008-9-4-r73 The electronic version of this article is the complete one and can be found online at http 2008 9 4 R73 Received 15 October 2007 Revised 14 February 2008 Accepted 17 April 2008 2008 Haussmann et al. licensee BioMed Central Ltd. This is an open access article distributed under the terms of the Creative Commons Attribution License http licenses by which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Abstract Background Formation of synaptic connections is a dynamic and highly regulated process. Little is known about the gene networks that regulate synaptic growth and how they balance stimulatory and restrictive signals. Results Here we show that the neuronally expressed transcription factor gene erect wing ewg is a major target of the RNA binding protein ELAV and that EWG restricts synaptic growth at neuromuscular junctions. Using a functional genomics approach we demonstrate that EWG acts primarily through increasing mRNA levels of genes involved in transcriptional and post-transcriptional regulation of gene expression while genes at the end of the regulatory expression hierarchy effector genes represent only a minor portion indicating an extensive regulatory network. Among EWG-regulated genes are components of Wingless and Notch signaling pathways. In a clonal analysis we demonstrate that EWG genetically interacts with Wingless and Notch and also with TGF-B and AP-1