tailieunhanh - Atomic Force Microscopy in Cell Biology Episode 2 Part 10

Tham khảo tài liệu 'atomic force microscopy in cell biology episode 2 part 10', kỹ thuật - công nghệ, cơ khí - chế tạo máy phục vụ nhu cầu học tập, nghiên cứu và làm việc hiệu quả | 366 Zuccheri and Samori between this class of methods and that presented here is that the latter is designed to specifically adsorb properly functionalized molecules. Often the protocols also reduce the non specific adsorption of other molecules in solution Bamdad 1998 . Such careful design makes the methods generally complex involving multistep functionalizations and works on a properly designed and modified target molecule with the exception of that presented by Shlyakhtenko and co-workers 1999 . For the general application in biology a deposition method usually cannot modify the molecules it is interested in because it is practically too difficult not to alter their properties. Due to the widespread interest of DNA attachment to surfaces for many techniques and uses DNA chip technology only to mention one it is certain that these methods will continue to evolve. The researcher will soon be offered a variety of methods to employ for the solution of any research problem. III. Air Imaging of DNA Which Present Which Future Imaging dehydrated specimens with the microscope operating in air is the easiest of the SFM operations. This is indeed one of the reasons for the popularity of the microscope and one of the advantages in its use with respect to the electron microscope which must always be used under high vacuum. In our experience any new user can master operations in ambient air reasonably well after a few hours of lecture in class and some hours of hands-on experience with the microscope. In the last few years the stability of the microscopes operating for instance in tapping mode see following and the constant quality of the commercial probes make imaging in air significantly easier than it was in the past. A. The Humidity Issue and SFM under Organic Solvents The control of the interaction forces between the probe and the specimen is of fundamental importance in SFM especially in contact-mode SFM the first to be employed during the years. In contact mode the .