tailieunhanh - Atomic Force Microscopy in Cell Biology Episode 2 Part 6

Tham khảo tài liệu 'atomic force microscopy in cell biology episode 2 part 6', kỹ thuật - công nghệ, cơ khí - chế tạo máy phục vụ nhu cầu học tập, nghiên cứu và làm việc hiệu quả | 286 Muller and Engel absorbance Fig. 19 Structural changes of photobleached purple membrane. A Absorption spectra of wild-type purple membrane solid line and of purple membrane photobleached in hydroxylamine to 10 and to 100 dashed lines . B Topograph of the cytoplasmic surface of purple membrane photobleached to 10 . C Cytoplasmic surface of purple membrane photobleached to 100 . A single bacteriorhodopsin trimer is outlined in the raw data. The inset represents the correlation average of the bacteriorhodopsin trimer. D Cytoplasmic surface of purple membrane fully regenerated after the addition of retinal chromophore. The bacteriorhodopsin trimers reassembled into the 2D crystal of purple membrane. The inset represents the correlation average of the cytoplasmic surface with the bacteriorhodopsin trimer located at the center of the image. All images were recorded in buffer solution 150 mM KCl 10 mM Tris-HCl pH at a loading force of 100 pN. Vertical full gray level range nm images courtesy of Clemens Moller University of Basel . 13. Atomic Force Microscopy and Spectroscopy of Membrane Proteins 287 IX. Detecting Intra- and Intermolecular Forces ofProteins A. Unzipping Single Protomers from a Bacterial Pore Structural chemical and morphological studies have shown that S layers are one of the most primitive membrane structures developed during evolution. They cover the cell surface completely are usually composed of a single protein and are endowed with the ability to assemble into monomolecular arrays by an entropy-driven process Sleytr et al. 1993 . Experimental results indicate that the integrity of the S-layer lattice is maintained by different combinations of weak bonds which are stronger than those binding to the underlying cell envelope component Sleytr 1997 Sleytr et al. 1993 . The inner surface of the S layer of Deinoccocus radiodurans the inner surface of the HPI layer is directly attached to the underlying outer cell membrane Fig. 15 via strong .