tailieunhanh - Báo cáo khoa học: The intracellular region of the Notch ligand Jagged-1 gains partial structure upon binding to synthetic membranes

Notch ligands are membrane-spanning proteins made of a large extracellu-lar region, a transmembrane segment, and a 100–200 residue cytoplasmic tail. The intracellular region of Jagged-1, one of the five ligands to Notch receptors in man, mediates protein–protein interactions through the C-ter-minal PDZ binding motif, is involved in receptor⁄ligand endocytosis trig-gered by mono-ubiquitination, | ỊFEBS Journal The intracellular region of the Notch ligand Jagged-1 gains partial structure upon binding to synthetic membranes Matija Popovic Alfredo De Biasio Alessandro Pintar and Sandor Pongor Protein Structure and Bioinformatics Group InternationalCentre for Genetic Engineering and Biotechnology ICGEB Padriciano Trieste Italy Keywords membrane cytoplasm interface regulated intramembrane proteolysis SDS micelles phospholipid vesicles in-cellNMR Correspondence A. Pintar and S. Pongor Protein Structure and Bioinformatics Group International Centre for Genetic Engineering and Biotechnology ICGEB AREA Science Park Padriciano 99 I-34012 Trieste Italy Fax 39 040226555 Tel 39 0403757354 E-mail pintar@ pongor@ Received 15 June 2007 revised 8 August 2007 accepted 20 August 2007 doi Notch ligands are membrane-spanning proteins made of a large extracellular region a transmembrane segment and a 100-200 residue cytoplasmic tail. The intracellular region of Jagged-1 one of the five ligands to Notch receptors in man mediates protein-protein interactions through the C-ter-minal PDZ binding motif is involved in receptor ligand endocytosis triggered by mono-ubiquitination and as a consequence of regulated intramembrane proteolysis can be released into the cytosol as a signaling fragment. The intracellular region of Jagged-1 may then exist in at least two forms as a membrane-tethered protein located at the interface between the membrane and the cytoplasm and as a soluble nucleocytoplasmic protein. Here we report the characterization in different environments of a recombinant protein corresponding to the human Jagged-1 intracellular region J1_tmic . In solution J1_tmic behaves as an intrinsically disordered protein but displays a significant helical propensity. In the presence of SDS micelles and phospholipid vesicles used to mimick the interface between the plasma membrane and the cytosol J1_tmic undergoes a substantial .

• Báo cáo khoa học
• Report medicine
• traditional medicine
• scientific reports
• molecular Biology
• tumor cells
• Antimicrobial
• Nelson’s pediatric antimicrobial therapy
• Choosing among antifungal agents
• Antimicrobial therapy for newborns
• Antimicrobial therapy according
• Clinical syndromes
• Beta lactamase inhibitors
• Antimicrobial therapy
• Mycobacterial pathogens
• Specific bacterial
• Nosocomial infection
• Hospital acquired infection
• Prevalence and antimicrobial susceptibility pattern
• Antimicrobial susceptibility pattern
• Evaluate antimicrobial susceptibility pattern
• Antimicrobial properties
• Antimicrobial activity
• Phyo chemical analysis
• Plant resources having antimicrobial properties obtained
• Phytochemical analysis of plant resources
• Antimicrobial activity of ZITRITIDE
• Organic antimicrobial fogging solution
• Special reference
• Infection prevention
• Hospital environments
• Other clean room facilities
• Specific parasitic pathogens
• Antimicrobial susceptibility
• Changing antimicrobial susceptibility pattern
• Pus samples
• Susceptibility pattern
• Antimicrobial susceptibility testing
• BMC Veterinary Research
• Antimicrobial usage
• Antimicrobial resistance
• Salmonella spp
• Alphabetic listing of antimicrobials
• Adverse reactions to antimicrobial agents
• Drug interactions
• Antibiotic therapy
• Alphabetic listing
• Symptomatic infection
• Antimicrobial prophylaxis
• Adverse reactions
• Lecture Microbiology
• Roles of microbes
• Biological research
• Antimicrobial drug properties
• Drug target sites
• Journal of Chemistry
• The Chemistry and Antimicrobial Activity of Psychotria reevesii Wall
• The Chemistry and Antimicrobial Activity
• Psychotria reevesii Wall
• The polar fractions against Staphylococcus aureus
• Journal of medical research
• Prevalence of antimicrobial resistance of neisseria gonorrhoeae
• Antimicrobial resistance of neisseria gonorrhoeae
• National hospital of dermatology and venereology in 2017
• Minimum inhibitory concentration
• Antimicrobial compound
• Burkholderia paludis
• Enterococcus faecalis
• The role of reactive oxygen species
• Pentafluorophenylammonium triflate
• Coumarin 3 carboxylic acid
• Chromeno[2 3 d]pyrimidinones
• 4H pyran 5 ethylcarboxylate
• Green synthesis
• Characterization and antimicrobial evaluation
• Antimicrobial evaluation
• Nano silver
• Mastitis causing microorganisms
• Bacterial profile
• Ventilator associated pneumonia
• BMC Pediatrics
• Pediatric community acquired pneumonia
• Community acquired pneumonia
• Empiric antimicrobial choice
• Heightened antimicrobial stewardship
• Virulence genes
• Identification and antimicrobial resistance
• Enterococcus faecalis isolated
• Diabetic foot patients
• ESKAPE pathogens
• Multidrug resistant (MDR) bacteria
• Tertiary care centre
• Antimicrobial susceptibility profile
• Clinical importance
• Staphylococcus aureus
• Hadman farm dairies
• Antimicrobial susceptibility test
• Cheese from Hadman farm dairies
• BMC Pharmacology and Toxicology
• MDR Salmonella
• Antimicrobial resistant Salmonella
• Pan resistance
• Drug sensitivity
• Amhara National Regional State
• Clinical microbiology laboratories
• Risk factors
• Mount Sinai Beth Israel
• Outpatient parenteral antimicrobial therapy
• Bacillus firmicutes
• Biopeptide or Bacterio cin
• Food borne pathogens