tailieunhanh - Báo cáo y học: "Lifting the veil on the transcriptome"

Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học Critical Care giúp cho các bạn có thêm kiến thức về ngành y học đề tài: Lifting the veil on the transcriptome. | Minireview Lifting the veil on the transcriptome Kevin P Callahan and J Scott Butler Addresses Departments of Biochemistry and Biophysics and Microbiology and Immunology University of Rochester Medical Center Elmwood Avenue Rochester NY 14642 USA. Correspondence J Scott Butler. Email btlr@ Published 24 April 2008 Genome Biology 2008 9 218 doi gb-2008-9-4-218 The electronic version of this article is the complete one and can be found online at http 2008 9 4 218 2008 BioMed Central Ltd Abstract Inhibition of the cellular RNA surveillance system in Arabidopsis thaliana results in the accumulation of thousands of transcripts arising from annotated and unannotated regions of the genome. This normally hidden transcriptome is replete with noncoding RNAs with the potential to regulate wide-ranging physiological activities. The eukaryotic genome was once thought to be collinear with defined regulatory regions controlling the initiation of transcription of their respective downstream proteincoding regions. Recent genome-wide analyses demand a revision of this view by revealing a genomic architecture now best described as interleaved and modular 1 . Tiling microarray analyses have identified thousands of RNAs arising from annotated and unannotated regions of eukaryotic genomes with transcription often occurring on both strands of the same region of DNA. Some of the RNAs produced from these noncoding regions appear to play a physiological role in the cell but the details remain obscure. In a recent paper in Cell Belostotsky and colleagues Chekanova et al. 2 now reveal an additional layer of complexity to the transcriptome of Arabidopsis thaliana that appears upon inhibition of the exosome a component of the RNA surveillance system Figure 1 . They have found novel species of RNA whose regulated expression may control critical physiological processes in eukaryotes. In addition they show that inhibition of RNA surveillance causes .