tailieunhanh - Báo cáo y học: " Increased serum soluble Fas after major trauma is associated with delayed neutrophil apoptosis and development of sepsis"

Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học Critical Care giúp cho các bạn có thêm kiến thức về ngành y học đề tài: Increased serum soluble Fas after major trauma is associated with delayed neutrophil apoptosis and development of sepsis. | Paunel-Gorgulu et al. Critical Care 2011 15 R20 http content 15 1 R20 KS CRITICAL CARE RESEARCH Open Access Increased serum soluble Fas after major trauma is associated with delayed neutrophil apoptosis and development of sepsis Adnana Paunel-Gorgulu Sascha Flohé Martin Scholz Joachim Windolf Tim Logters Abstract Introduction Deregulated apoptosis and overshooting neutrophil functions contribute to immune and organ dysfunction in sepsis and multiple organ failure MOF . In the present study we determined the role of soluble Fas sFas in the regulation of posttraumatic neutrophil extrinsic apoptosis and the development of sepsis. Methods Forty-seven major trauma patients 18 with and 29 without sepsis development during the first 10 days after trauma were enrolled in this prospective study. Seventeen healthy volunteers served as controls. Blood samples from severely injured patients were analyzed at day 1 day 5 and day 9 after major trauma. sFas levels plasma levels of neutrophil elastase PMNE and levels of interleukin IL -6 were quantified by enzyme-linked immunosorbent assay and related to patients Sequential Organ Failure Assessment SOFA score and Multiple Organ Dysfunction Score MODS . Neutrophil apoptosis was determined by propidium iodide staining of fragmented DNA and flow cytometry. sFas-mediated effects on neutrophil apoptosis were investigated in cells cultured with agonistic anti-Fas antibodies in the presence of recombinant sFas sFas-depleted serum or untreated serum from septic patients. Results Serum levels of sFas in patients who later developed sepsis were significantly increased at day 5 P and day 9 P after trauma compared with patients with uneventful recovery. Apoptosis of patient neutrophils was significantly decreased during the observation period compared with control cells. Moreover Fas-mediated apoptosis of control neutrophils was efficiently inhibited by recombinant sFas and serum from septic patients. Depletion of sFas .