tailieunhanh - Báo cáo khoa học: Triterpene synthases from the Okinawan mangrove tribe, Rhizophoraceae

Oleanane-type triterpene is one of the most widespread triterpenes found in plants, together with the lupane type, and these two types often occur together in the same plant. Bruguiera gymnorrhiza(L.) Lamk. and Rhizo-phora stylosaGriff. (Rhizophoraceae) are known to produce both types of triterpenes. | ễFEBS Journal Triterpene synthases from the Okinawan mangrove tribe Rhizophoraceae Mohammad Basyuni1 Hirosuke Oku2 Etsuko Tsujimoto3 Kazuhiko Kinjo3 Shigeyuki Baba4 and Kensaku Takara3 1 United Graduate Schoolof AgriculturalSciences Kagoshima University Japan 2 Division of Molecular Biotechnology Center of Molecular Biosciences University of the Ryukyus Okinawa Japan 3 Faculty of Agriculture University of the Ryukyus Okinawa Japan 4 TropicalBiosphere Research Center University of the Ryukyus Okinawa Japan Keywords Bruguiera gymnorrhiza L. Lamk. mangrove molecular evolution oxidosqualene cyclases Rhizophora stylosa Griff Correspondence H. Oku Division of Molecular Biotechnology Center of Molecular Biosciences University of the Ryukyus Nishihara Okinawa 903-0213 Japan Fax 81 98 895 8972 Tel 81 98 895 8972 E-mail okuhiros@ Database The nucleotide sequences reported in the present study have been submitted to the DDBJ EMBL GenBank databases under the accession numbers AB263203 RsM1 AB263204 RsM2 AB289585 BgbAS and AB289586 BgLUS Received 5 June 2007 revised 26 July 2007 accepted 31 July 2007 doi Oleanane-type triterpene is one of the most widespread triterpenes found in plants together with the lupane type and these two types often occur together in the same plant. Bruguiera gymnorrhiza L. Lamk. and Rhizo-phora stylosa Griff. Rhizophoraceae are known to produce both types of triterpenes. Four oxidosqualene cyclase cDNAs were cloned from the leaves of B. gymnorrhiza and R. stylosa by a homology-based PCR method. The ORFs of full-length clones termed BgbAS 2280 bp coding for 759 amino acids BgLUS 2286 bp coding for 761 amino acids RsM1 2280 bp coding for 759 amino acids and RsM2 2316 bp coding for 771 amino acids were ligated into yeast expression plasmid pYES2 under the control of the GAL1 promoter. Expression of BgbAS and BgLUS in GIL77 resulted in the production of b-amyrin and lupeol suggesting that these genes .