tailieunhanh - Báo cáo khoa học: Construction and biological activity of a full-length molecular clone of human Torque teno virus (TTV) genotype 6

Torque teno virus (TTV) is a non-enveloped human virus with a circular negative-sense (approximately 3800 nucleotides) ssDNA genome. TTV resembles in genome organization the chicken anemia virus, the animal pathogen of theCircoviridaefamily, and is currently classified as a member of a new, floating genus,Anellovirus. | ỊFEBS Journal Construction and biological activity of a full-length molecular clone of human Torque teno virus TTV genotype 6 Laura Kakkola1 Johanna Tommiska1 Linda C. L. Boele2 Simo Miettinen1 Tea Blom1 Tuija Kekarainen2 Jianming Qiu3 David Pintel3 Rob C. Hoeben2 Klaus Hedman1 and Maria Soderlund-Venermo1 1 Department of Virology Haartman Institute and Helsinki University Central Hospital University of Helsinki Finland 2 Department of Molecular Cell Biology Leiden University MedicalCenter the Netherlands 3 Department of Molecular Microbiology and Immunology University of Missouri-Columbia Life Sciences Center Columbia MO USA Keywords Anellovirus replication Torque teno virus transcription Correspondence L. Kakkola Department of Virology Haartman Institute Haartmaninkatu 3 PO Box 21 University of Helsinki FIN-00014 Finland Fax 358 9 19126491 Tel 358 9 19126676 E-mail Website http english Note Nucleotide sequence data are available in the DDBJ EMBL GenBank databases under the accession number AY666122 Received 11 April2007 revised 10 July 2007 accepted 11 July 2007 doi Torque teno virus TTV is a non-enveloped human virus with a circular negative-sense approximately 3800 nucleotides ssDNA genome. TTV resembles in genome organization the chicken anemia virus the animal pathogen of the Circoviridae family and is currently classified as a member of a new floating genus Anellovirus. Molecular and cell biological research on TTV has been restricted by the lack of permissive cell lines and functional replication-competent plasmid clones. In order to examine the key biological activities . RNA transcription and DNA replication of this still poorly characterized ssDNA virus we cloned the full-length genome of TTV genotype 6 and transfected it into cells of several types. TTV mRNA transcription was detected by RT-PCR in all the cell types KU812Ep6 Cos-1 293 293T Chang liver Huh7 and UT7 Epo-S1. .