tailieunhanh - Báo cáo khoa học: "Three-dimensional Huh7 cell culture system for the study of Hepatitis C virus infection"

Three-dimensional Huh7 cell culture system for the study of Hepatitis C virus infection | Virology Journal BioMed Central Research Three-dimensional Huh7 cell culture system for the study of Hepatitis C virus infection Bruno Sainz Jr 1 Veronica TenCate 1 and Susan L Uprichard 1 2 Open Access Address Department of Medicine The University of Illinois at Chicago Chicago IL 60612 USA and 2Department of Microbiology and Immunology The University of Illinois at Chicago Chicago IL 60612 USA Email Bruno Sainz - bsainz@ Veronica TenCate - vtencate@ Susan L Uprichard - sluprich@ Corresponding author fEqual contributors Published 15 July 2009 Received 13 June 2009 Virology Journal 2009 6 103 doi 1743-422X-6-103 Accepted 15 July 2009 This article is available from http content 6 1 103 2009 Sainz et al licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License http licenses by which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Abstract Background In order to elucidate how Hepatitis C Virus HCV interacts with polarized hepatocytes in vivo and how HCV-induced alterations in cellular function contribute to HCV-associated liver disease a more physiologically relevant hepatocyte culture model is needed. As such NASA-engineered three-dimensional 3-D rotating wall vessel RWV bioreactors were used in effort to promote differentiation of HCV-permissive Huh7 hepatoma cells. Results When cultured in the RWV Huh7 cells became morphologically and transcriptionally distinct from more standard Huh7 two-dimensional 2-D monolayers. Specifically RWV-cultured Huh7 cells formed complex multilayered 3-D aggregates in which Phase I and Phase II xenobiotic drug metabolism genes as well as hepatocyte-specific transcripts HNF4a Albumin TTR and a1AT were upregulated compared to 2-D cultured Huh7 cells. Immunofluorescence analysis revealed that these HCV-permissive 3-D cultured Huh7