tailieunhanh - Báo cáo khoa học: The importance of polymerization and galloylation for the antiproliferative properties of procyanidin-rich natural extracts

Grape (Vitis vinifera) and pine (Pinus pinaster) bark extracts are widely used as nutritional supplements. Procyanidin-rich fractions from grape and pine bark extract showing different mean degrees of polymerization, per-centage of galloylation (percentage of gallate esters) and reactive oxygen species-scavenging capacity were tested on HT29 human colon cancer cells. | ễFEBS Journal The importance of polymerization and galloylation for the antiproliferative properties of procyanidin-rich natural extracts D. Lizarraga1 C. Lozano2 J. J. Briedé3 J. H. van Delft3 S. Tourino2 J. J. Centelles1 J. L. Torres2 and M. Cascante1 2 1 Biochemistry and Molecular Biology Department Biology Faculty University of Barcelona Biomedicine Institute from University of Barcelona IBUB and Centre for Research in TheoreticalChemistry Scientific Park of Barcelona CeRQT-PCB Associated Unit to CSIC Spain 2 Institute for Chemicaland EnvironmentalResearch IIQAB-CSIC Barcelona Spain 3 Department of Health Risk Analysis and Toxicology Maastricht University the Netherlands Keywords antiproliferative apoptosis cell cycle colon cancer scavenger capacity Correspondence M. Cascante Serratosa Department of Biochemistry and Molecular Biology University of Barcelona Biology Faculty Av. Diagonal645 08028 Barcelona Spain Fax 34 934021219 Tel 34 934021593 E-mail martacascante@ Received 2 May 2007 revised 3 July 2007 accepted 18 July 2007 Grape Vitis vinifera and pine Pinus pinaster bark extracts are widely used as nutritional supplements. Procyanidin-rich fractions from grape and pine bark extract showing different mean degrees of polymerization percentage of galloylation percentage of gallate esters and reactive oxygen species-scavenging capacity were tested on HT29 human colon cancer cells. We observed that the most efficient fractions in inhibiting cell proliferation arresting the cell cycle in G2 phase and inducing apoptosis were the grape fractions with the highest percentage of galloylation and mean degree of polymerization. Additionally the antiproliferative effects of grape fractions were consistent with their oxygen radical-scavenging capacity and their ability to trigger DNA condensation-fragmentation. doi Colorectal cancer is the third most commonly diagnosed cancer in the world and is one of the major causes of .