tailieunhanh - Báo cáo y học: " Lack of effect of glutamine administration to boost the innate immune system response in trauma patients in the intensive care unit"

Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học Critical Care giúp cho các bạn có thêm kiến thức về ngành y học đề tài: Lack of effect of glutamine administration to boost the innate immune system response in trauma patients in the intensive care unit. | Pérez-Bárcena et al. Critical Care 2010 14 R233 http content 14 6 R233 KS CRITICAL CARE RESEARCH Open Access Lack of effect of glutamine administration to boost the innate immune system response in trauma patients in the intensive care unit 3 -4 1 1 1 Jon Pérez-Bárcena Catalina Crespí Veronica Regueiro Pedro Marsé Joan M Raurich Jordi Ibanez Abelardo García de Lorenzo-Mateos2 5 José A Bengoechea4 Abstract Introduction The use of glutamine as a dietary supplement is associated with a reduced risk of infection. We hypothesized that the underlying mechanism could be an increase in the expression and or functionality of Toll-like receptors TLR key receptors sensing infections. The objective of this study was to evaluate whether glutamine supplementation alters the expression and functionality of TLR2 and TLR4 in circulating monocytes of trauma patients admitted to the intensive care unit ICU . Methods We designed a prospective randomized and single-blind study. Twenty-three patients received parenteral nutrition TPN with a daily glutamine supplement of g kg. The control group 20 patients received an isocaloric-isonitrogenated TPN. Blood samples were extracted before treatment at 6 and 14 days. Expression of TLR2 and TLR4 was determined by flow cytometry. Monocytes were stimulated with TLR specific agonists and cytokines were measured in cell culture supernatants. Phagocytic ability of monocytes was also determined. Results Basal characteristics were similar in both groups. Monocytes from patients treated with glutamine expressed the same TLR2 levels as controls before treatment rmfi vs. rmfi respectively P at Day 6 rmfi vs. rmfi respectively P and at Day 14 rfim vs. rmfi respectively P . TLR4 levels were not significantly different between the groups before treatment 1 rmfi vs rmfi respectively P at Day 6 1 rmfi vs. rmfi respectively P and at Day 14