tailieunhanh - Báo cáo khoa học: " Characterization of the RNA-binding properties of the triple-gene-block protein 2 of Bamboo mosaic virus"

Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành y học dành cho các bạn tham khảo đề tài: Characterization of the RNA-binding properties of the triple-gene-block protein 2 of Bamboo mosaic virus | Virology Journal BioMed Central Open Access Short report Characterization of the RNA-binding properties of the triple-gene-block protein 2 of Bamboo mosaic virus Hsiu-Ting Hsu1 Yang-Hao Tseng1 Yuan-Lin Chou1 Shiaw-Hwa Su2 Yau-Heiu Hsu 3 and Ban-Yang Chang 1 Address Institute of Biochemistry National Chung-Hsing University Taichung 40227 Taiwan PR China 2Department of Soil and Environmental Science National Chung-Hsing University Taichung 40227 Taiwan PR China and 3Graduate Institute of Biotechnology National Chung-Hsing University Taichung 40227 Taiwan PR China Email Hsiu-Ting Hsu - hsuhsiutingh@ Yang-Hao Tseng - wallace1114@ Yuan-Lin Chou - sppchou@ Shiaw-Hwa Su - spsu@ Yau-Heiu Hsu - yhhsu@ Ban-Yang Chang - bychang@ Corresponding authors Published 7 May 2009 Received 5 March 2009 Virology Journal 2009 6 50 doi 1743-422X-6-50 Accepted 7 May 2009 This article is available from http content 6 1 50 2009 Hsu et al licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License http licenses by which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Abstract The triple-gene-block protein 2 TGBp2 of Bamboo mosaic virus BaMV is a transmembrane protein which was proposed to be involved in viral RNA binding during virus transport. Here we report on the RNA-binding properties of TGBp2. Using tyrosine fluorescence spectroscopy and UV-crosslinking assays the TGBp2 solubilized with Triton X-100 was found to interact with viral RNA in a non-specific manner. These results raise the possibility that TGBp2 facilitates intracellular delivery of viral RNA through non-specific protein-RNA interaction. Findings Bamboo mosaic virus BaMV is a single-stranded positivesense RNA virus. Its genomic RNA has three partially .