tailieunhanh - Báo cáo khoa học: " Profile of time-dependent VEGF upregulation in human pulmonary endothelial cells, HPMEC-ST1.6R infected with DENV-1, -2, -3, and -4 viruses"

Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành y học dành cho các bạn tham khảo đề tài: Profile of time-dependent VEGF upregulation in human pulmonary endothelial cells, infected with DENV-1, -2, -3, and -4 viruses | Virology Journal BioMed Central Open Access Profile of time-dependent VEGF upregulation in human pulmonary endothelial cells infected with DENV-1 -2 -3 and -4 viruses Azliyati Azizan 1 Kelly Fitzpatrick2 4 Aimee Signorovitz2 Richard Tanner1 Heidi Hernandez2 Lillian Stark1 2 and Mark Sweat1 Address 1Global Health Department College of Public Health 13201 Bruce B Downs Bvld Tampa Florida 33612 USA and 2Florida Department of Health Bureau of Laboratory 3602 Spectrum Blvd Tampa Florida 33612 USA Email Azliyati Azizan - aazizan@ Kelly Fitzpatrick - kfitzpat@ Aimee Signorovitz - Signarovitz@ Richard Tanner - Tanner@ Heidi Hernandez - hahernan@ Lillian Stark - Lillian_Stark@ Mark Sweat - jsweat@ Corresponding author Published 6 May 2009 Received 19 March 2009 Virology Journal 2009 6 49 doi 1743-422X-6-49 Accepted 6 May 2009 This article is available from http content 6 l 49 2009 Azizan et al licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License http licenses by which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Abstract In this study the upregulated expression level of vascular endothelial growth factor VEGF in a pulmonary endothelial cell line infected with dengue virus serotypes 1 2 3 and 4 DENV-1 -2 -3 and -4 was investigated. This cell line exhibits the major constitutive and inducible endothelial cell characteristics as well as angiogenic response. Infection by all four DENV serotypes was confirmed by an observed cytopathic effect CPE as well as RT-PCR reverse- transcription polymerase chain reaction assays. As we had previously reported the DENV-infected cells exhibited an elongated cytoplasmic morphology possibly representing a response to .