tailieunhanh - Báo cáo khoa học: "Development and Validation of a HPV-32 Specific PCR Assay"

Development and Validation of a HPV-32 Specific PCR Assay | Virology Journal BioMed Central Open Access Development and Validation of a HPV-32 Specific PCR Assay Nicholas R Herrel1 Nadia L Johnson2 Jennifer E Cameron4 Janet Leigh3 and Michael E Hagensee 2 Address Department of Microbiology Immunology and Parasitology Louisiana State University Health Sciences Center New Orleans USA 2Department of Medicine Louisiana State University Health Sciences Center New Orleans USA 3Department of Oral Medicine Louisiana State University Health Sciences Center New Orleans USA and 4Cancer Center Tulane University Health Sciences Center New Orleans USA Email Nicholas R Herrel - nherre@ Nadia L Johnson - nadia13@ Jennifer E Cameron - jcamero1@ Janet Leigh - jleigh@ Michael E Hagensee - mhagen@ Corresponding author Published 27 June 2009 Received 2 June 2009 Virology Journal 2009 6 90 doi 1743-422X-6-90 Accepted 27 June 2009 This article is available from http content 6 1 90 2009 Herrel et al licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License http licenses by which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Abstract Background Human Papillomavirus-32 HPV-32 has traditionally been associated with focal-epithelial-hyperplasia FEH . It is also present in 58 of oral warts of HIV-positive individuals whose prevalence is increasing. Current methods for the detection of HPV-32 are labor-intensive and insensitive so the goal of this work was to develop a highly sensitive and easy to use specific polymerase chain reaction PCR assay. Materials and methods An HPV-32 L1 specific PCR assay was developed and optimized. The sensitivity and specificity was compared to previous assays utilized for detection PGMY and MY09 11 PCR with dot blot hybridization using cloned HPV-32 L1 the closely related HPV-42 L1