tailieunhanh - Báo cáo khoa học: Presence of necrotic strains of Potato virus Y in Mexican potatoes

Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành y học dành cho các bạn tham khảo đề tài: Presence of necrotic strains of Potato virus Y in Mexican potatoes | Virology Journal BioMed Central Research Presence of necrotic strains of Potato virus Y in Mexican potatoes Victoriano Roberto Ramirez-Rodriguez1 Katia Avina-Padilla1 Gustavo Frias- Trevino2 Laura Silva-Rosales1 and Juan Pablo Martinez-Soriano 1 Open Access Address 1Centro de Investigación y de Estudios Avanzados del Institute Politécnico Nacional Campus Guanajuato km. libramiento norte carretera Irapuato-León 36821 Irapuato Guanajuato Mexico and 2Universidad Autónoma Agraria Antonio Narro Departamento de Parasitologia Buenavista Saltillo Coahuila Mexico Email Victoriano Roberto Ramirez-Rodriguez - vicr358@ Katia Avina-Padilla - kap@ Gustavo Frias-Trevino - gfriast@ Laura Silva-Rosales - lsilva@ Juan Pablo Martinez-Soriano - jpms@ Corresponding author Published 6 May 2009 Received II March 2009 Virology Journal 2009 6 48 doi I743-422X-6-48 Accepted 6 May 2009 This article is available from http content 6 1 48 2009 Ramirez-Rodriguez et al licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License http licenses by which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Abstract_ As part of a routine screening for the possible presence of the necrotic strains of potato virus Y affecting potatoes in Mexico five PVY isolates were submitted to biological and molecular analysis. Considering their serological properties two belong to the common strain O and three to the necrotic strain N . All the isolates induced vein necrosis in Nicotiana tabacum. To characterize the isolates 5 NTR and pi genes were sequenced and compared with sequences from GenBank database. Nucleotide sequence similarity ranged from 47-100 in the 5 NTR and from 63-100 in the