tailieunhanh - Báo cáo khoa học: "Vaccinia virus p37 interacts with host proteins associated with LE-derived transport vesicle biogenesis"

Vaccinia virus p37 interacts with host proteins associated with LE-derived transport vesicle biogenesis | Virology Journal BioMed Central Research Vaccinia virus p37 interacts with host proteins associated with LE-derived transport vesicle biogenesis Yali Chen Kady M Honeychurch Guang Yang Chelsea M Byrd Chris Harver Dennis E Hruby and Robert Jordan Open Access Address SIGA Technologies Inc Corvallis Oregon 97333 USA Email Yali Chen - ychen@ Kady M Honeychurch - khoneychurch@ Guang Yang - gyang@ Chelsea M Byrd - cbyrd@ Chris Harver - charver@ Dennis E Hruby - dhruby@ Robert Jordan - rjordan@ Corresponding author Published 28 April 2009 Received 16 March 2009 Accepted 28 April 2009 Virology Journal 2009 6 44 doi 1743-422X-6-44 This article is available from http content 6 1 44 2009 Chen et al licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License http licenses by which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Abstract Background Proteins associated with the late endosome LE appear to play a central role in the envelopment of a number of taxonomically diverse viruses. How viral proteins interact with LE-associated proteins to facilitate envelopment is not well understood. LE-derived transport vesicles form through the interaction of Rab9 GTPase with cargo proteins and TIP47 a Rab9-specific effector protein. Vaccinia virus VV induces a wrapping complex derived from intracellular host membranes to envelope intracellular mature virus particles producing egress-competent forms of virus. Results We show that VV p37 protein associates with TIP47- Rab9- and CI-MPR-containing membranes. Mutation of a di-aromatic motif in p37 blocks association with TIP47 and inhibits plaque formation. ST-246 a specific inhibitor of p37 function inhibits these interactions and also blocks wrapped virus particle formation. Vaccinia virus .