tailieunhanh - Báo cáo khoa học: " Short hairpin-loop-structured oligodeoxynucleotides reduce HSV-1 replication"

Short hairpin-loop-structured oligodeoxynucleotides reduce HSV-1 replication | Virology Journal BioMed Central Open Access Short report Short hairpin-loop-structured oligodeoxynucleotides reduce HSV-1 replication Alexander Falkenhagen1 Jochen Heinrich1 and Karin Moelling 1 2 Address University of Zurich Gloriastrasse 30 32 CH-8006 Zurich Switzerland and institute for Advanced Study Wallotstrafie 19 D-14193 Berlin Germany Email Alexander Falkenhagen - Jochen Heinrich - jheinric@ Karin Moelling - moelling@ Corresponding author Published 27 April 2009 Received 25 March 2009 Accepted 27 April 2009 Virology journal 2009 6 43 doi 1743-422X-6-43 This article is available from http content 6 1 43 2009 Falkenhagen et al licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License http licenses by which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Abstract The Herpes simplex virus HSV is known as an infectious agent and widespread in the human population. The symptoms of HSV infections can range from mild to life threatening especially in immune-compromised individuals. HSV infections are commonly treated with the guanosine analogue Aciclovir but reports of resistance are increasing. Efforts are made to establish singlestranded antisense oligodeoxynucleotides as and small interfering ribonucleic acids siRNAs for antiviral treatment. Recently another class of short interfering nucleic acids partially doublestranded hairpin loop-structured 54 mer oligodeoxynucleotides ODNs was shown to allow hydrolysis of HIV RNA by binding to the viral RNA. This leads to a substrate for the viral RNase H. To assess the potential of such ODNs for inhibition of HSV-1 replication five partially doublestranded ODNs were designed based on the sequences of known siRNAs against HSV-1 with antiviral activity. Three of them are directed against