tailieunhanh - Báo cáo khoa học: "Dengue viruses binding proteins from Aedes aegypti and Aedes polynesiensis salivary glands"

Dengue viruses binding proteins from Aedes aegypti and Aedes polynesiensis salivary glands | Virology Journal BioMed Central Open Access Short report Dengue viruses binding proteins from Aedes aegypti and Aedes polynesiensis salivary glands Van-Mai Cao-Lormeau Address Head Laboratoire de Recherche en Virologie Médicale Institut Louis Malardé Po Box 30 98 713 Papeete Tahiti French Polynesia Email Van-Mai Cao-Lormeau - mlormeau@ Published 25 March 2009 Received 8 October 2008 Accepted 25 March 2009 Virology Journal 2009 6 35 doi l 743-422X-6-35 This article is available from http content 6 1 35 2009 Cao-Lormeau licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License http licenses by which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Abstract Dengue virus DENV the etiological agent of dengue fever is transmitted to the human host during blood uptake by an infective mosquito. Infection of vector salivary glands and further injection of infectious saliva into the human host are key events of the DENV transmission cycle. However the molecular mechanisms of DENV entry into the mosquito salivary glands have not been clearly identified. Otherwise although it was demonstrated for other vector-transmitted pathogens that insect salivary components may interact with host immune agents and impact the establishment of infection the role of mosquito saliva on DENV infection in human has been only poorly documented. To identify salivary gland molecules which might interact with DENV at these key steps of transmission cycle we investigated the presence of proteins able to bind DENV in salivary gland extracts SGE from two mosquito species. Using virus overlay protein binding assay we detected several proteins able to bind DENV in SGE from Aedes aegypti L. and Aedes polynesiensis Marks . The present findings pave the way for the identification of proteins mediating DENV .