tailieunhanh - Báo cáo khoa học: " Development of TaqMan® MGB fluorescent real-time PCR assay for the detection of anatid herpesvirus 1"

Tham khảo luận văn - đề án 'báo cáo khoa học: " development of taqman® mgb fluorescent real-time pcr assay for the detection of anatid herpesvirus 1"', luận văn - báo cáo phục vụ nhu cầu học tập, nghiên cứu và làm việc hiệu quả | Virology Journal BioMed Central Research Development of TaqMan MGB fluorescent real-time PCR assay for the detection of anatid herpesvirus 1 Yufei Guo1 2 Anchun Cheng 1 2 Mingshu Wang 1 2 Chanjuan Shen2 Renyong Jia1 Shun Chen1 and Na Zhang1 Address 1Avian Disease Research Center College of Veterinary Medicine Sichuan Agricultural University Yaan 625014 PR China and 2Key Laboratory of Animal Diseases and Human Health of Sichuan Province Sichuan Agricultural University Yaan 625014 PR China Email Yufei Guo - gyf02@ Anchun Cheng - chenganchun@ Mingshu Wang - mshwang@ Chanjuan Shen - vober@ Renyong Jia - cqrc_jry@ Shun Chen - sophia_cs@ Na Zhang - nana821024@ Corresponding authors Open Access Published 4 June 2009 Received 6 April 2009 Accepted 4 June 2009 Virology Journal 2009 6 71 doi l 743-422X-6-71 This article is available from http content 6 1 71 2009 Guo et al licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License http licenses by which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Abstract Background Anatid herpesvirus 1 AHV-1 is an alphaherpesvirus associated with latent infection and mortality in ducks and geese and is currently affecting the world-wide waterfowl production severely. Here we describe a fluorescent quantitative real-time PCR FQ-PCR method developed for fast measurement of AHV-1 DNA based on TaqMan MGB technology. Results The detection limit of the assay was 1 X 101 standard DNA copies with a sensitivity of 2 logs higher than that of the conventional gel-based PCR assay targeting the same gene. The realtime PCR was reproducible as shown by satisfactory low intra-assay and inter-assay coefficients of variation. Conclusion The high sensitivity specificity simplicity and reproducibility of the AHV-1 .