tailieunhanh - Báo cáo khoa học: "Improvement of a real-time RT-PCR assay for the detection of enterovirus RNA"

Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành y học dành cho các bạn tham khảo đề tài: Improvement of a real-time RT-PCR assay for the detection of enterovirus RNA | Virology Journal BioMed Central Open Access Short report Improvement of a real-time RT-PCR assay for the detection of enterovirus RNA Marian AC Piqueur Walter A Verstrepen Peggy Bruynseels and An H Mertens Address Department of Microbiology ZNA Hospitals site Middelheim Lindendreef 1 2020 Antwerp Belgium Email Marian AC Piqueur - marianpiqueur@ Walter A Verstrepen - Peggy Bruynseels - An H Mertens - Corresponding author Published 7 July 2009 Received 26 May 2009 Virology Journal 2009 6 95 doi 1743-422X-6-95 Accepted 7 July 2009 This article is available from http content 6 1 95 2009 Piqueur et al licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License http licenses by which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Abstract We describe an improvement of an earlier reported real-time RT-PCR assay for the detection of enterovirus RNA based on the 5 exonuclease digestion of a dual-labeled fluorogenic probe by Taq DNA polymerase. A different extraction method real-time RT-PCR instrument and primer set were evaluated. Our data show that the optimized assay yields a higher sensitivity and reproducibility and resulted in a significant reduced hands-on time per sample. Findings Enteroviruses are responsible for a substantial number of aseptic meningitis and encephalitis especially in neonates and infants 1 2 . In recent years a number of rapid diagnostic tests for enterovirus have been developed including different RT-PCR assays 3 4 . Our study group earlier reported a real-time RT-PCR assay for the detection of enterovirus RNA based on the 5 exonuclease digestion of a dual-labeled fluorogenic probe by Taq DNA polymerase. This assay has now been further improved by comparing different extraction .