tailieunhanh - Báo cáo khoa học: " Hepatitis C virus core protein induces apoptosis-like caspase independent cell death"

Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành y học dành cho các bạn tham khảo đề tài: Hepatitis C virus core protein induces apoptosis-like caspase independent cell death | Virology Journal BioMed Central Research Hepatitis C virus core protein induces apoptosis-like caspase independent cell death Christoph P Berg Stephan F Schlosser Dorothee KH Neukirchen Costa Papadakis Michael Gregor Sebastian Wesselborg and Gerburg M Stein Open Access Address Department of Internal Medicine I Medical Clinic University of Tubingen Germany Email Christoph P Stephan F Dorothee KH Neukirchen - neukirchen@ Costa Papadakis - kopa77@ Michael Gregor - Sebastian Wesselborg - Gerburg M Stein - Corresponding author Published I December 2009 Received 3 August 2009 Accepted 1 December 2009 Virology Journal 2009 6 213 doi 1743-422X-6-21 3 This article is available from http content 6 1 213 2009 Berg et al licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License http licenses by which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Abstract Background Hepatitis C virus HCV associated liver diseases may be related to apoptotic processes. Thus we investigated the role of different HCV proteins in apoptosis induction as well as their potency to interact with different apoptosis inducing agents. Methods and Results The use of a tightly adjustable tetracycline Tet -dependent HCV protein expression cell system with the founder osteosarcoma cell line U-2 OS allowed switch-off and on of the endogenous production of HCV proteins. Analyzed were cell lines expressing the HCV polyprotein the core protein protein complexes of the core envelope proteins El E2 and p7 and non-structural proteins NS3 and NS4A NS4B or NS5A and NS5B. Apoptosis was measured mainly by the detection of hypodiploid .