tailieunhanh - Báo cáo khoa học: " The construction and characterization of the bi-directional promoter between pp38 gene and 1.8-kb mRNA transcripts of Marek's disease viruses"

Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành y học dành cho các bạn tham khảo đề tài: The construction and characterization of the bi-directional promoter between pp38 gene and mRNA transcripts of Marek's disease viruses | Virology Journal BioMed Central Research The construction and characterization of the bi-directional promoter between pp38 gene and mRNA transcripts of Marek s disease viruses Ruiai Chen1 2 Jiabo Ding 3 and Bin Wang 1 Open Access Address College of Biological Sciences China Agricultural University Beijing 100193 China 2Guangdong Dahuanong Animal Health Products LTD Xinxing Guangdong 527400 China and 3China Institute of Veterinary Drug Control Beijing 100081 China Email Ruiai Chen - chensa727@ Jiabo Ding - dingjiabo@ Bin Wang - bwang03@ Corresponding authors Published 30 November 2009 Received 14 October 2009 Accepted 30 November 2009 Virology Journal 2009 6 212 doi l743-422X-6-2l 2 This article is available from http content 6 l 2l2 2009 Chen et al licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License http licenses by which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Abstract Background Marek s disease virus MDV has a bi-directional promoter between pp38 gene and mRNA transcripts. By sequencing for the promoters from 8 different strains CVI988 814 GA JM Md5 G2 RBlB and 648A it is found comparing with the other 7 MDV strains CVI988 has a 5-bp from -628 to -632 deletion in this region which caused a Spl site destroyed. In order to analysis the activity of the promoter the complete bi-directional promoters from GA and CVI988 were respectively cloned into pCAT-Basic vector in both directions for the recombinants pPGA pp38 -CAT pPGA kb -CAT pPCV pp38 -CAT and pPCVI kb -CAT. The complete promoter of GA was divided into two single-direction promoters from the replication of MDV genomic DNA and cloned into pCAT-Basic for pdPGA pp38 -CAT and .