tailieunhanh - Báo cáo y học: " Characterization of probiotic Escherichia coli isolates with a novel pan-genome microarray"

Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học Critical Care giúp cho các bạn có thêm kiến thức về ngành y học đề tài: Characterization of probiotic Escherichia coli isolates with a novel pan-genome microarray. | Open Access Characterization of probiotic Escherichia coli isolates with a novel pan-genome microarray Hanni Willenbrock Peter F Hallin Trudy M Wassenaar and David W Ussery Addresses Center for Biological Sequence Analysis Technical University of Denmark 2800 Lyngby Denmark. tExiqon A S 2950 Vedbffik Denmark. Molecular Microbiology and Genomics Consultants Tannenstrasse 55576 Zotzenheim Germany. Correspondence Hanni Willenbrock. Email hanni@ Published 18 December 2007 Genome Biology 2007 8 R267 doi gb-2007-8- l2-r267 The electronic version of this article is the complete one and can be found online at http 2007 8 12 R267 Received 30 July 2007 Revised 4 October 2007 Accepted l8 December 2007 2008 Willenbrock et al. licensee BioMed Central Ltd. This is an open access article distributed under the terms of the Creative Commons Attribution License http licenses by which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Abstract Background Microarrays have recently emerged as a novel procedure to evaluate the genetic content of bacterial species. So far microarrays have mostly covered single or few strains from the same species. However with cheaper high-throughput sequencing techniques emerging multiple strains of the same species are rapidly becoming available allowing for the definition and characterization of a whole species as a population of genomes - the pan-genome . Results Using 32 Escherichia coli and Shigella genome sequences we estimate the pan- and core genome of the species. We designed a high-density microarray in order to provide a tool for characterization of the E. coli pan-genome. Technical performance of this pan-genome microarray based on control strain samples E. coli K-12 and O157 H7 demonstrated a high sensitivity and relatively low false positive rate. A single-channel analysis approach is robust while allowing the .