tailieunhanh - Báo cáo y học: " Model-based analysis of two-color arrays (MA2C)"

Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học Critical Care giúp cho các bạn có thêm kiến thức về ngành y học đề tài: Model-based analysis of two-color arrays (MA2C). | Method Open Access Model-based analysis of two-color arrays MA2C Jun S SongH W Evan Johnson Xiaopeng ZhuH Xinmin Zhang Wei Li Arjun K Manrai Jun S Liu Runsheng Chen and X Shirley Liu Addresses Department of Biostatistics and Computational Biology Dana-Farber Cancer Institute 44 Binney Street Boston MA 02115 USA. Department of Biostatistics Harvard School of Public Health Boston MA 02115 USA. Bioinformatics Laboratory Institute of Biophysics Chinese Academy of Sciences Beijing 100101 China. NimbleGen Systems Inc. Science Court Madison Wisconsin 53711 USA. Department of Physics Harvard University Cambridge MA 02138 USA. Department of Statistics Harvard University 1 Oxford Street Cambridge MA 02138 USA. H These authors contributed equally to this work. Correspondence X Shirley Liu. Email xsliu@ Published 29 August 2007 Genome Biology 2007 8 RI78 doi gb-2007-8-8-rl 78 The electronic version of this article is the complete one and can be found online at http 2007 8 8 R178 Received 20 April 2007 Revised 2 July 2007 Accepted 29 August 2007 2007 Song et al. licensee BioMed Central Ltd. This is an open access article distributed under the terms of the Creative Commons Attribution License http licenses by which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Abstract A novel normalization method based on the GC content of probes is developed for two-color tiling arrays. The proposed method together with robust estimates of the model parameters is shown to perform superbly on published data sets. A robust algorithm for detecting peak regions is also formulated and shown to perform well compared to other approaches. The tools have been implemented as a stand-alone Java program called MA2C which can display various plots of statistical analysis for quality control. Background High-density oligonucleotide tiling-microarrays currently provide the .