tailieunhanh - Báo cáo khoa học: " Bioinformatic evidence for a stem-loop structure 5'-adjacent to the IGR-IRES and for an overlapping gene in the bee paralysis dicistroviruses"

Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành y học dành cho các bạn tham khảo đề tài: Bioinformatic evidence for a stem-loop structure 5'-adjacent to the IGR-IRES and for an overlapping gene in the bee paralysis dicistroviruses | Virology Journal BioMed Central Open Access Short report Bioinformatic evidence for a stem-loop structure 5 -adjacent to the IGR-IRES and for an overlapping gene in the bee paralysis dicistroviruses Andrew E Firth 1 Qing S Wang2 Eric Jan2 and John F Atkins 1 3 Address 1BioSciences Institute University College Cork Cork Ireland 2Department of Biochemistry and Molecular Biology University of British Columbia 2350 Health Sciences Mall Vancouver BC Canada and 3Department of Human Genetics University of Utah Salt Lake City UT 841125330 USA Email Andrew E Firth - Qing S Wang - qing1@ Eric Jan - ej@ John F Atkins - Corresponding authors Published 6 November 2009 Received 23 September 2009 Accepted 6 November 2009 Virology Journal 2009 6 193 doi 1743-422X-6-193 This article is available from http content 6 1 193 2009 Firth et al licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License http licenses by which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Abstract__ The family Dicistroviridae order Picornavirales includes species that infect insects and other arthropods. These viruses have a linear positive-sense ssRNA genome of 8-10 kb which contains two long ORFs. The 5 ORF encodes the nonstructural polyprotein while the 3 ORF encodes the structural polyprotein. The dicistroviruses are noteworthy for the intergenic Internal Ribosome Entry Site IGR-IRES that mediates efficient translation initation on the 3 ORF without the requirement for initiator Met-tRNA. Acute bee paralysis virus Israel acute paralysis virus of bees and Kashmir bee virus form a distinct subgroup within the Dicistroviridae family. In this brief report we describe the