tailieunhanh - Báo cáo y học: "Contribution of telomerase RNA retrotranscription to DNA double-strand break repair during mammalian genome evolution"

Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học Critical Care giúp cho các bạn có thêm kiến thức về ngành y học đề tài: Contribution of telomerase RNA retrotranscription to DNA double-strand break repair during mammalian genome evolution. | Research Open Access Contribution of telomerase RNA retrotranscription to DNA double-strand break repair during mammalian genome evolution Solomon G Nergadze Marco Andrea Santagostino Alberto Salzano Chiara Mondello and Elena Giulotto Addresses Dipartimento di Genetica e Microbiologia Adriano Buzzati-Traverso Università degli Studi di Pavia Via Ferrata 27100 Pavia Italy. dstituto di Genetica Molecolare CNR Via Abbiategrasso 27100 Pavia Italy. Correspondence Elena Giulotto. Email Published 7 December 2007 Genome Biology 2007 8 R260 doi gb-2007-8- l2-r260 The electronic version of this article is the complete one and can be found online at http 2007 8 12 R260 Received 4 October 2007 Revised 28 November 2007 Accepted 7 December 2007 2008 Nergadze et al. licensee BioMed Central Ltd. This is an open access article distributed under the terms of the Creative Commons Attribution License http licenses by which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Abstract Background In vertebrates tandem arrays of TTAGGG hexamers are present at both telomeres and intrachromosomal sites interstitial telomeric sequences ITSs . We previously showed that in primates ITSs were inserted during the repair of DNA double-strand breaks and proposed that they could arise from either the capture of telomeric fragments or the action of telomerase. Results An extensive comparative analysis of two primate Homo sapiens and Pan troglodytes and two rodent Mus musculus and Rattus norvegicus genomes allowed us to describe organization and insertion mechanisms of all the informative ITSs present in the four species. Two novel observations support the hypothesis of telomerase involvement in ITS insertion in a highly significant fraction of informative loci the ITSs were introduced at break sites where a few nucleotides homologous to the telomeric hexamer were .