tailieunhanh - Báo cáo khoa học: " Inhibition of Monkeypox virus replication by RNA interference"

Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành y học dành cho các bạn tham khảo đề tài: Inhibition of Monkeypox virus replication by RNA interference | Virology Journal BioMed Central Research Inhibition of Monkeypox virus replication by RNA interference Abdulnaser Alkhalil 1 Sarah Strand1 Eric Mucker1 John W Huggins1 Peter B Jahrling2 and Sofi M Ibrahim1 Open Access Address 1United States Army Medical Research Institute of Infectious Diseases Fort Detrick MD 21702 USA and 2Emerging Viral Pathogens Section National Institute of Allergy and Infectious Diseases National Institutes of Health Bethesda MD 20894 USA Email Abdulnaser Alkhalil - Sarah Strand - Eric Mucker - muckerEM@ John W Huggins - hugginsJW@ Peter B Jahrling - Sofi M Ibrahim - Corresponding author Published 4 November 2009 Received II September 2009 Accepted 4 November 2009 Virology Journal 2009 6 188 doi 1743-422X-6-188 This article is available from http content 6 1 188 2009 Alkhalil et al licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License http licenses by which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Abstract The Orthopoxvirus genus of Poxviridae family is comprised of several human pathogens including cowpox CPXV Vaccinia VACV monkeypox MPV and Variola VARV viruses. Species of this virus genus cause human diseases with various severities and outcome ranging from mild conditions to death in fulminating cases. Currently vaccination is the only protective measure against infection with these viruses and no licensed antiviral drug therapy is available. In this study we investigated the potential of RNA interference pathway RNAi as a therapeutic approach for orthopox virus infections using MPV as a model. Based on genome-wide expression studies and bioinformatic analysis we selected 12 viral genes and targeted