tailieunhanh - Báo cáo y học: "Genomic mapping of Suppressor of Hairy-wing binding sites in Drosophila"

Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học Critical Care giúp cho các bạn có thêm kiến thức về ngành y học đề tài:Genomic mapping of Suppressor of Hairy-wing binding sites in Drosophila. | Open Access Research Genomic mapping of Suppressor of Hairy-wing binding sites in Drosophila Boris Adryan Gertrud Woerfel Ian Birch-Machin Shan Gao Marie Quick Lisa Meadows Steven Russell and Robert White Addresses Department of Physiology Development and Neuroscience University of Cambridge Downing Street Cambridge CB2 3DY UK. Theoretical and Computational Biology Group MRC Laboratory of Molecular Biology Hills Road Cambridge CB2 0QH UK. Department of Genetics University of Cambridge Downing Street Cambridge CB2 3EH UK. Correspondence Robert White. Email rw108@ Published 16 August 2007 Received 20 July 2007 Genome Biology 2007 8 R167 doi gb-2007-8-8-r167 Accepted 16 August 2007 The electronic version of this article is the complete one and can be found online at http 2007 8 8 R167 2007 Adryan et al. licensee BioMed Central Ltd. This is an open access article distributed under the terms of the Creative Commons Attribution License http licenses by which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Abstract Background Insulator elements are proposed to play a key role in the organization of the regulatory architecture of the genome. In Drosophila one of the best studied is the gypsy retrotransposon insulator which is bound by the Suppressor of Hairy-wing Su Hw transcriptional regulator. Immunolocalization studies suggest that there are several hundred Su Hw sites in the genome but few of these endogenous Su Hw binding sites have been identified. Results We used chromatin immunopurification with genomic microarray analysis to identify in vivo Su Hw binding sites across the 3 megabase Adh region. We find 60 sites and these enabled the construction of a robust new Su Hw binding site consensus. In contrast to the gypsy insulator which contains tightly clustered Su Hw binding sites endogenous sites generally occur as isolated sites. These .