tailieunhanh - Báo cáo y học: "The roles of binding site arrangement and combinatorial targeting in microRNA repression of gene expression"

Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học Critical Care giúp cho các bạn có thêm kiến thức về ngành y học đề tài:The roles of binding site arrangement and combinatorial targeting in microRNA repression of gene expression. | Research Open Access The roles of binding site arrangement and combinatorial targeting in microRNA repression of gene expression Lawrence S Hon and Zemin Zhang Address Department of Bioinformatics Genentech Inc. 1 DNA Way South San Francisco CA 94080 USA. Correspondence Zemin Zhang. Email Published 14 August 2007 Genome Biology 2007 8 RI66 doi gb-2007-8-8-rl 66 The electronic version of this article is the complete one and can be found online at http 2007 8 8 R166 Received 18 June 2007 Revised 30 July 2007 Accepted I4 August 2007 2007 Hon and Zhang licensee BioMed Central Ltd. This is an open access article distributed under the terms of the Creative Commons Attribution License http licenses by which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Abstract Background MicroRNAs miRNAs are small noncoding RNAs that bind mRNA target transcripts and repress gene expression. They have been implicated in multiple diseases such as cancer but the mechanisms of this involvement are not well understood. Given the complexity and degree of interactions between miRNAs and target genes understanding how miRNAs achieve their specificity is important to understanding miRNA function and identifying their role in disease. Results Here we report factors that influence miRNA regulation by considering the effects of both single and multiple miRNAs targeting human genes. In the case of single miRNA targeting we developed a metric that integrates miRNA and mRNA expression data to calculate how changes in miRNA expression affect target mRNA expression. Using the metric our global analysis shows that the repression of a given miRNA on a target mRNA is modulated by 3 untranslated region length the number of target sites and the distance between a pair of binding sites. Additionally we show that some miRNAs preferentially repress transcripts with longer .